This paper presents the experimental analysis for the fluctuation of rolling friction force, which deteriorates high precision motion in linear ball guideways. A simple drive with four tracks and four balls was used to investigate the influence of contact surface and contact load on the friction force fluctuation. To provide different surface integrity, ground, polished, and shot-peened surfaces were prepared. Experimental results showed that the friction force fluctuation was dependent on position change and influenced by the inclination angle of the rolling contact surface. The fluctuation of friction coefficient was inversely proportional to the cube root of the contact load. The wavelength analysis on the friction coefficient showed that friction coefficient was suppressed as the contact load increased. An empirical formula for estimating the friction force fluctuation was proposed based on these experimental results and a single ball friction model proposed by Ishikawa and Suda.