論文

査読有り 国際誌
2020年6月

Neutralizing antibody against osteopontin attenuates non-alcoholic steatohepatitis in mice.

Journal of cell communication and signaling
  • Machiko Honda
  • ,
  • Chiemi Kimura
  • ,
  • Toshimitsu Uede
  • ,
  • Shigeyuki Kon

14
2
開始ページ
223
終了ページ
232
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s12079-020-00554-7

Previously, we reported that an extracellular matrix protein, osteopontin (OPN), is involved in various autoimmune diseases using a neutralizing polyclonal antibody against OPN generated in rabbits. However, the antibody cannot be used for long-term mouse models of chronic inflammatory disease because of the induction of antibodies against anti-OPN rabbit IgG. In this study, we generated a new antibody, anti-mouse OPN mouse IgG (35B6). 35B6 inhibited the cell adhesion of mouse and human OPN to Chinese Hamster Ovary (CHO) cells or CHO cells expressing α4 or α9 integrin. It was reported that OPN is highly expressed and has an important role in a chronic liver disease, non-alcoholic steatohepatitis (NASH). 35B6 injection twice a week for 8 weeks attenuated liver inflammation and fibrosis in a NASH mouse model, suggesting 35B6 is beneficial for the treatment of NASH. 35B6 was preferable to the rabbit anti-OPN antibody for investigating the in vivo function of OPN in mouse models of long-term disease.

リンク情報
DOI
https://doi.org/10.1007/s12079-020-00554-7
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32062834
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7272532

エクスポート
BibTeX RIS