論文

査読有り 国際誌
2019年8月15日

Syndapin constricts microvillar necks to form a united rhabdomere in Drosophila photoreceptors.

Development (Cambridge, England)
  • Sakiko Ogi
  • ,
  • Atsushi Matsuda
  • ,
  • Yuna Otsuka
  • ,
  • Ziguang Liu
  • ,
  • Takunori Satoh
  • ,
  • Akiko K Satoh

146
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記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1242/dev.169292

Drosophila photoreceptors develop from polarized epithelial cells that have apical and basolateral membranes. During morphogenesis, the apical membranes subdivide into a united bundle of photosensory microvilli (rhabdomeres) and a surrounding supporting membrane (stalk). By EMS-induced mutagenesis screening, we found that the F-Bin/Amphiphysin/Rvs (F-BAR) protein syndapin is essential for apical membrane segregation. The analysis of the super-resolution microscopy, STORM and the electron microscopy suggest that syndapin localizes to the neck of the microvilli at the base of the rhabdomere. Syndapin and moesin are required to constrict the neck of the microvilli to organize the membrane architecture at the base of the rhabdomere, to exclude the stalk membrane. Simultaneous loss of syndapin along with the microvilli adhesion molecule chaoptin significantly enhanced the disruption of stalk-rhabdomere segregation. However, loss of the factors involving endocytosis do not interfere. These results indicated syndapin is most likely functioning through its membrane curvature properties, and not through endocytic processes for stalk-rhabdomere segregation. Elucidation of the mechanism of this unconventional domain formation will provide novel insights into the field of cell biology.

リンク情報
DOI
https://doi.org/10.1242/dev.169292
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31371377
ID情報
  • DOI : 10.1242/dev.169292
  • ISSN : 0950-1991
  • PubMed ID : 31371377

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