論文

国際誌
2019年5月

Higher lactate and purine metabolite levels in erythrocyte-rich fresh venous thrombus: Potential markers for early deep vein thrombosis.

Thrombosis research
  • Kazunari Maekawa
  • ,
  • Chihiro Sugita
  • ,
  • Atsushi Yamashita
  • ,
  • Sayaka Moriguchi-Goto
  • ,
  • Eiji Furukoji
  • ,
  • Tatefumi Sakae
  • ,
  • Toshihiro Gi
  • ,
  • Toshinori Hirai
  • ,
  • Yujiro Asada

177
開始ページ
136
終了ページ
144
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.thromres.2019.03.011

BACKGROUND: Thrombolytic therapy is effective in fresh deep vein thrombosis (DVT) although the benefit may fall below the risk of bleeding in non-fresh thrombosis. Markers reflecting fresh DVT have not been established. The present study aims to identify metabolites reflecting fresh venous thrombus and their role in thrombus formation. METHODS: Metabolites of rabbit venous blood and jugular venous thrombus 4 h after thrombus induction were analysed using electrophoresis-time of flight mass spectrometry. The effects of the altered metabolites on blood coagulation and platelet aggregation were assessed by using rotation thromboelastometry and platelet aggregometer. Cellular contents and glucose transporter (Glut)-1 expression in aspirated human DVT samples were pathologically analysed. RESULTS: Metabolome analysis identified 226 metabolites (133 cationic and 93 anionic metabolites). Largely altered 18 metabolites (thrombus/blood ratio: >5 or <0.5) included glycolytic metabolites, redox-related metabolites, purine nucleotides and tryptophan metabolites. Among the metabolites with >5-fold increase, lactic acid was most abundant and guanine modestly enhanced whole blood clotting with thromboelastometry. Lactic acid and adenosine monophosphate inhibited collagen-induced platelet aggregation. Human DVTs were rich in erythrocytes expressing Glut-1. The erythrocyte content and Glut-1 expression were negatively correlated with the time after onset of DVT. CONCLUSIONS: Glycolysis-, purine-, and redox-related metabolites may reflect fresh erythrocyte-rich venous thrombus, and altered metabolites may affect venous thrombus formation. An increased level of lactate may reflect active glycolysis of thrombus cellular components, predominantly erythrocytes.

リンク情報
DOI
https://doi.org/10.1016/j.thromres.2019.03.011
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30901608
ID情報
  • DOI : 10.1016/j.thromres.2019.03.011
  • PubMed ID : 30901608

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