論文

査読有り 国際誌
2017年2月

Simultaneous measurement of monoamine metabolites and 5-methyltetrahydrofolate in the cerebrospinal fluid of children.

Clin Chim Acta
  • Akiyama Tomoyuki
  • ,
  • Hayashi Yumiko
  • ,
  • Hanaoka Yoshiyuki
  • ,
  • Shibata Takashi
  • ,
  • Akiyama Mari
  • ,
  • Nakamura Kazuyuki
  • ,
  • Tsuyusaki Yu
  • ,
  • Kubota Masaya
  • ,
  • Yoshinaga Harumi
  • ,
  • Kobayashi Katsuhiro

465
開始ページ
5
終了ページ
10
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.cca.2016.12.005

BACKGROUND: We describe a new method for simultaneous measurement of monoamine metabolites (3-O-methyldopa [3-OMD], 3-methoxy-4-hydroxyphenylethyleneglycol [MHPG], 5-hydroxyindoleacetic acid [5-HIAA], and homovanillic acid [HVA]) and 5-methyltetrahydrofolate (5-MTHF) and its use on cerebrospinal fluid (CSF) samples from pediatric patients. METHODS: Monoamine metabolites and 5-MTHF were measured by high-performance liquid chromatography with fluorescence detection. CSF samples were prospectively collected from children according to a standardized collection protocol in which the first 1-ml fraction was used for analysis. RESULTS: Monoamine metabolites and 5-MTHF were separated within 10min. They showed linearity from the limit of detection to 1024nmol/l. The limit of quantification of each metabolite was sufficiently low for the CSF sample assay. In 42 CSF samples after excluding cases with possibly altered neurotransmitter profiles, the concentrations of 3-OMD, MHPG, 5-HIAA, HVA, and 5-MTHF showed significant age dependence and their ranges were comparable with the reference values in the literature. The metabolite profiles of aromatic l-amino acid decarboxylase deficiency, Segaw

リンク情報
DOI
https://doi.org/10.1016/j.cca.2016.12.005
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27940130
ID情報
  • DOI : 10.1016/j.cca.2016.12.005
  • ISSN : 1873-3492
  • PubMed ID : 27940130

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