論文

査読有り
2008年5月

Expression of Fgf15 is regulated by both activator and repressor forms of Gli2 in vitro

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • Munekazu Komada
  • ,
  • Hirotomo Saitsu
  • ,
  • Kohei Shiota
  • ,
  • Makoto Ishibashi

369
2
開始ページ
350
終了ページ
356
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbrc.2008.02.015
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

Fibroblast growth factor 15 (Fgf15) is expressed in the medial region of diencephalon and midbrain by the seven-somite stage. In the previous studies, we showed that Sonic hedgehog signaling through Gli protein is required for Fgf15 expression in this region. The Fgf15 expression domain overlapped with that of Gli2 and the Gli-binding site (GliBs) is located in the 3.6-kb 5'-flanking enhancer/promoter region of the Fgf15 gene. In this study, we identified the two additional Gli-binding sites in row, called Gli-responsive elements (GliREs). Chromatin immunoprecipitation assay indicated that Gli2 directly binds to GliREs. The results from luciferase assays indicated that the Gli2 activator form binds to the GliBS and that the Gli2 repressor form binds to the GliREs. These findings suggest that the repressor form of Gli2 preferentially binds to the GliREs to control Fgf15 expression. (c) 2008 Elsevier Inc. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2008.02.015
CiNii Articles
http://ci.nii.ac.jp/naid/80019444665
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/18279667
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000254676900014&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.bbrc.2008.02.015
  • ISSN : 0006-291X
  • CiNii Articles ID : 80019444665
  • PubMed ID : 18279667
  • Web of Science ID : WOS:000254676900014

エクスポート
BibTeX RIS