2018年12月1日
Multiple entry pathways within the efflux transporter AcrB contribute to multidrug recognition
Nature Communications
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- 巻
- 9
- 号
- 1
- 開始ページ
- 124
- 終了ページ
- 記述言語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/s41467-017-02493-1
© 2018 The Author(s). AcrB is the major multidrug exporter in Escherichia coli. Although several substrate-entrances have been identified, the specificity of these various transport paths remains unclear. Here we present evidence for a substrate channel (channel 3) from the central cavity of the AcrB trimer, which is connected directly to the deep pocket without first passing the switch-loop and the proximal pocket. Planar aromatic cations, such as ethidium, prefer channel 3 to channels 1 and 2. The efflux through channel 3 increases by targeted mutations and is not in competition with the export of drugs such as minocycline and erythromycin through channels 1 and 2. A switch-loop mutant, in which the pathway from the proximal to the deep pocket is hindered, can export only channel 3-utilizing drugs. The usage of multiple entrances thus contributes to the recognition and transport of a wide range of drugs with different physicochemical properties.
- リンク情報
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- DOI
- https://doi.org/10.1038/s41467-017-02493-1
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/29317622
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85040629293&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85040629293&origin=inward
- ID情報
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- DOI : 10.1038/s41467-017-02493-1
- eISSN : 2041-1723
- PubMed ID : 29317622
- SCOPUS ID : 85040629293