論文

国際誌
2021年1月7日

PDGF-BB and IL-4 co-overexpression is a potential strategy to enhance mesenchymal stem cell-based bone regeneration.

Stem cell research & therapy
  • Ning Zhang
  • ,
  • Chi-Wen Lo
  • ,
  • Takeshi Utsunomiya
  • ,
  • Masahiro Maruyama
  • ,
  • Ejun Huang
  • ,
  • Claire Rhee
  • ,
  • Qi Gao
  • ,
  • Zhenyu Yao
  • ,
  • Stuart B Goodman

12
1
開始ページ
40
終了ページ
40
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1186/s13287-020-02086-8

BACKGROUND: Mesenchymal stem cell (MSC)-based therapy has the potential for immunomodulation and enhancement of tissue regeneration. Genetically modified MSCs that over-express specific cytokines, growth factors, or chemokines have shown great promise in pre-clinical studies. In this regard, the anti-inflammatory cytokine interleukin (IL)-4 converts pro-inflammatory M1 macrophages into an anti-inflammatory M2 phenotype; M2 macrophages mitigate chronic inflammation and enhance osteogenesis by MSC lineage cells. However, exposure to IL-4 prematurely inhibits osteogenesis of MSCs in vitro; furthermore, IL-4 overexpressing MSCs inhibit osteogenesis in vivo during the acute inflammatory period. Platelet-derived growth factor (PDGF)-BB has been shown to enhance osteogenesis of MSCs with a dose-dependent effect. METHODS: In this study, we generated a lentiviral vector that produces PDGF-BB under a weak promoter (phosphoglycerate kinase, PGK) and lentiviral vector producing IL-4 under a strong promoter (cytomegalovirus, CMV). We infected MSCs with PDGF-BB and IL-4-producing lentiviral vectors separately or in combination to investigate cell proliferation and viability, protein expression, and the capability for osteogenesis. RESULTS: PDGF-BB and IL-4 co-overexpression was observed in the co-infected MSCs and shown to enhance cell proliferation and viability, and osteogenesis compared to IL-4 overexpressing MSCs alone. CONCLUSIONS: Overexpression of PDGF-BB together with IL-4 mitigates the inhibitory effect of IL-4 on osteogenesis by IL-4 overexpressing MSCS. PDGF-BB and IL-4 overexpressing MSCs may be a potential strategy to facilitate osteogenesis in scenarios of both acute and chronic inflammation.

リンク情報
DOI
https://doi.org/10.1186/s13287-020-02086-8
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33413614
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7792350
ID情報
  • DOI : 10.1186/s13287-020-02086-8
  • PubMed ID : 33413614
  • PubMed Central 記事ID : PMC7792350

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