2011年3月
Biochemical and histopathological alterations in TAR DNA-binding protein-43 after acute ischemic stroke in rats
JOURNAL OF NEUROCHEMISTRY
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- 巻
- 116
- 号
- 6
- 開始ページ
- 957
- 終了ページ
- 965
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1111/j.1471-4159.2010.06860.x
- 出版者・発行元
- WILEY-BLACKWELL
P>Nuclear factor TAR DNA-binding protein-43 (TDP-43) is considered to play roles in pathogenesis of human neurodegenerative diseases, so-called TDP-43 proteinopathy, via its proteolytic cleavage, abnormal phosphorylation, subcellular redistribution, and insolubilization generating TDP-43-positive neuronal intracellular inclusions. The purpose of this study was to elucidate biochemical and histopathological alternations in TDP-43 specific to acute ischemic stroke. Adult male rats were subjected to a 90-min middle cerebral artery occlusion. We examined the proteolytic cleavage, phosphorylation, subcellular localization, and solubility of TDP-43 by immunoblottings and histopathological examinations using the ischemic and sham-operated cortex. The level of full-length TDP-43 (43 kDa) decreased and that of the 25-kDa C-terminal fragment increased after acute ischemic stroke, which can be explained by proteolytic cleavage of TDP-43. Cytoplasmic redistribution and altered nuclear distribution of TDP-43 was observed after acute ischemic stroke, whereas abnormal phosphorylation and insolubilization of TDP-43 as well as formation of intracellular inclusions were not observed. Ischemic neurons with the cytoplasmic redistribution of TDP-43 expressed ubiquitin and activated caspase 3 and were terminal deoxynucleotidyl transferase-mediated uridine 5'-triphosphate-biotin nick end labeling-positive. In conclusion, biochemical and histopathological alterations in TDP-43 were identified in rats after acute ischemic stroke, although there was very less similarity between TDP-43 alterations observed in acute ischemic stroke and those observed in TDP-43 proteinopathy.
- リンク情報
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- DOI
- https://doi.org/10.1111/j.1471-4159.2010.06860.x
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/20557425
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000287856200003&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?eid=2-s2.0-79952375490&partnerID=MN8TOARS
- URL
- http://orcid.org/0000-0001-6337-8156
- ID情報
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- DOI : 10.1111/j.1471-4159.2010.06860.x
- ISSN : 0022-3042
- ORCIDのPut Code : 28502358
- PubMed ID : 20557425
- SCOPUS ID : 79952375490
- Web of Science ID : WOS:000287856200003