論文

査読有り
2004年1月

PDZ domain-binding motif of human T-cell leukemia virus type 1 Tax oncoprotein augments the transforming activity in a rat fibroblast cell line

VIROLOGY
  • A Hirata
  • ,
  • M Higuchi
  • ,
  • A Niinuma
  • ,
  • M Ohashi
  • ,
  • M Fukushi
  • ,
  • M Oie
  • ,
  • T Akiyama
  • ,
  • Y Tanaka
  • ,
  • F Gejyo
  • ,
  • M Fujii

318
1
開始ページ
327
終了ページ
336
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.virol.2003.10.006
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

While human T-cell leukemia virus type 1 (HTLV-1) is associated with the development of adult T-cell leukemia (ATL), HTLV-2 has not been reported to be associated with such malignant leukemias. HTLV-1 Tax1 oncoprotein transforms a rat fibroblast cell line (Rat-1) to form multiple large colonies in soft agar, and this activity is much greater than that of HTLV-2 Tax2. We have demonstrated here that the increased number of transformed colonies induced by Tax 1 relative to Tax2 was mediated by a PDZ domain-binding motif (PBM) in Tax1, which is absent in Tax2. Taxi PBM mediated the interaction of Tax1 with the discs large (Dlg) tumor suppressor containing PDZ domains, and the interaction correlated well with the transforming activities of Tax1 and the mutants. Through this interaction, Taxi altered the subcellular localization of Diu from the detergent-soluble to the detergent-insoluble fraction in a fibroblast cell line as well as in HTLV-1-infected T-cell lines. These results suggest that the interaction of Tax1 with PDZ domain protein(s) is critically involved in the transforming activity of Tax1, the activity of which may be a crucial factor in malignant transformation of HTLV-1-infected cells in vivo. (C) 2003 Elsevier Inc. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.virol.2003.10.006
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/14972558
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000189243000031&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.virol.2003.10.006
  • ISSN : 0042-6822
  • PubMed ID : 14972558
  • Web of Science ID : WOS:000189243000031

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