論文

査読有り
2003年12月

Latency-associated nuclear antigen of Kaposi's sarcoma-associated herpesvirus interacts with human myeloid cell nuclear differentiation antigen induced by interferon alpha

VIRUS GENES
  • M Fukushi
  • ,
  • M Higuchi
  • ,
  • M Oie
  • ,
  • T Tetsuka
  • ,
  • F Kasolo
  • ,
  • K Ichiyama
  • ,
  • N Yamamoto
  • ,
  • H Katano
  • ,
  • T Sata
  • ,
  • M Fujii

27
3
開始ページ
237
終了ページ
247
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1023/A:1026391715071
出版者・発行元
KLUWER ACADEMIC PUBL

Kaposi's sarcoma-associated herpesvirus (KSHV)/human herpes virus type 8 (HHV-8) is tightly linked to the development of Kaposi's sarcoma, primary effusion lymphoma (PEL) and some cases of multicentric Castleman's disease. Latency-associated nuclear antigen (LANA) is one of a limited number of KSHV genes consistently expressed in these diseases as well as in KSHV-infected cell lines derived from PEL, and has been shown to play crucial role in persistence of KSHV genomes in the infected cells. In this study, we explored the cellular factors that interact with LANA using yeast two-hybrid screening, and isolated a part of gene encoding human myeloid cell nuclear differentiation antigen (MNDA). MNDA is a hematopoietic interferon-inducible nuclear proteins with a HIN-200 family member with conserved 200-amino acid repeats. Immunoprecipitation assay revealed that LANA interacted with MNDA in a mammalian embryonic kidney cell line. MNDA transcript was undetectable in three PEL cell lines by reverse-transcription polymerase chain reaction, but it was induced by interferon alpha (IFNalpha). Moreover, LANA and MNDA were co-localized in the nuclei of MNDA-expressing PEL cells. Our results suggest that LANA interacts with MNDA in KSHV-infected cells exposed to IFNalpha. Such interaction may modulate IFN-mediated host defense activities.

リンク情報
DOI
https://doi.org/10.1023/A:1026391715071
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/14618084
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000186265900004&DestApp=WOS_CPL
ID情報
  • DOI : 10.1023/A:1026391715071
  • ISSN : 0920-8569
  • PubMed ID : 14618084
  • Web of Science ID : WOS:000186265900004

エクスポート
BibTeX RIS