論文

査読有り 国際誌
2004年10月

Effect of tetrahydrobiopterin on nitric oxide synthase-containing cells in the rat hippocampus.

Neuroscience research
  • Kunio Koshimura
  • ,
  • Yoshio Murakami
  • ,
  • Junko Tanaka
  • ,
  • Masahiro Yamamoto
  • ,
  • Yuzuru Kato

50
2
開始ページ
161
終了ページ
7
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.neures.2004.06.012
出版者・発行元
ELSEVIER SCI IRELAND LTD

We have observed that tetrahydrobiopterin (BH4), a cofactor of nitric oxide synthase (NOS), acts as a self-protection factor against nitric oxide (NO) toxicity in PC12 cells. To further investigate the self-protection action of BH4 in vivo, the effect of deletion of endogenous BH4 on NO-producing cells was examined in the rat hippocampus. Following the peripheral infusion of 50 mM 2,4-diamino-6-hydroxypyrimidine (DAHP), an inhibitor of GTP cyclohydrolase I, using a miniosmotic pump for 14 days, BH4 content in the hippocampus decreased as compared with the control group administered with vehicle solution, which had no effect on brain BH4 content. When the rats were administered with 50 mM DAHP and 10 mM BH4, the DAHP-induced decrease in BH4 content was prevented. The extracellular concentration of NO metabolites remained unchanged following DAHP administration, suggesting that DAHP-induced decrease in BH4 content had no effect on NO production. The number of NOS-positive cells decreased following DAHP administration in the hippocampal regions, while the number of NOS-negative cells remained unchanged. The DAHP-induced decrease in the NOS-positive cell number was prevented by the administration of 10 mM BH4 and DAHP. These results suggest that endogenous BH4 may affect NOS-positive cell number in the rat hippocampus.

リンク情報
DOI
https://doi.org/10.1016/j.neures.2004.06.012
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/15380323
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000224597000003&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.neures.2004.06.012
  • ISSN : 0168-0102
  • PubMed ID : 15380323
  • Web of Science ID : WOS:000224597000003

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