FUJISHIMA Masahiro

J-GLOBAL         Last updated: Jun 17, 2019 at 18:21
 
Avatar
Name
FUJISHIMA Masahiro
Affiliation
Yamaguchi University
Section
Graduate School, of Science and Engineering, Department of Environmental Science and Engineering
Job title
Professor (specially designated)
Degree
Bachelor of Science(Hirosaki University), Master of Science(Tohoku University), Doctor of Science(Tohoku University)
Other affiliation
Yamaguchi University, Faculty of Science, Department of Chemistry and BiologyNational Bioresource Project Paramecium
Twitter ID
@mfujishim

Profile

The ciliated protista, Paramecium species, are extremely valuable cells that enable the reestablishment experiments of the endosymbiosis, which frequently bear prokaryotic, eukaryotic, or both endosymbionts in the cell. Although most endosymbiotic bacteria of Paramecium species cannot grow outside the host cell as a result of their reduced genome size, endonuclear symbiotic bacteria Holospora species, even when isolated from the host cells, can maintain their infectivity to new host cell for few days at room temperature. Although the host can acquire various stress resistances by endosymbiosis with Holospora, the symbionts are not indispensable for the host’s survival. Consequently, reestablishment of endosymbiosis between the symbiotic bacteria-free Paramecium and Holospora cells isolated from the host cells can be induced easily through the host phagocytosis by mixing them.

On the other hand, among Paramecium species, only P. bursaria and P. chlorelligerum have the capacity to harbor endosymbiotic green algae in the cytoplasm. Irrespective of mutual relationship between P. bursaria and the symbiotic algae, both cells still retain the ability to grow without the partner, and can easily reestablish endosymbiosis by mixing them. Thus, interactions between Paramecium species and Holospora species, and between P. bursaria or P. chlorelligerum and symbiotic green algae provide excellent opportunities to elucidate not only for control mechanisms for establishment of primary symbiosis, but also for secondary symbiosis leading to eukaryotic cell evolution.

Our research is focused on
1. How does the symbiont invade the host cytoplasm?
2. How can the symbiont avoid digestion by the host's lysosomal enzymes?
3. How can the symbiont grow synchronously with the host cell?
4. How and when is the horizontal gene transfer from symbiont to the host genome induced?
5. What benefit does the host cell receive by endosymbiosis to stabilize endosymbiosis and to adapt to new environments for expanding the range that the hosts can live in?

Research Areas

 
 

Academic & Professional Experience

 
Apr 1997
 - 
Mar 2016
Professor, Graduate Scool of Science and Engineering, Yamaguchi University
 
Oct 1995
 - 
Mar 2002
Professor, Graduate Scool of Science, Tokyo University
 
Jul 1992
 - 
Mar 1997
Professor, Faculty of Science, Yamaguchi University
 
Jul 1984
 - 
Jun 1992
Associate Professor, Faculty of Science, Yamaguchi University
 
May 1979
 - 
Jul 1984
Assistant Professor, Faculty of Science, Yamaguchi University
 
Apr 1979
 - 
Apr 1979
Research Fellow of The Japan Society for the Promotion of Science, Graduate School of Science, Tohoku University
 
Apr 1979
 - 
Apr 1979
Research Fellow, The Japan Society for the Promotion of Science
 
 
   
 
      Faculty of Science, Yamaguchi University
 
 
   
 
      University of Tokyo
 
1979
 - 
1984
Assistant, Faculty of Science,
 
Oct 1980
 - 
Sep 1981
Research Fellow of Alexander von Humboldt Foundation, Zoological Institute, Muenster University
 
1984
 - 
1992
Associate Professor,
 
Aug 1988
 - 
Sep 1988
Visiting Research Fellow, Zoological Institute, Hong Kong University,
 
1992
   
 
-    Professor, Faculty of Science,
 
1995
   
 
-    Professor, Graduate School of Science,
 
Nov 2004
 - 
Mar 2006
Director, Reserarch Institure of Times Syudies, Yamaguchi University
 
Apr 2016
 - 
Today
Professor (Specially designated), Graduate School of Sciences and Technology for Innovation,       Yamaguchi University
 

Education

 
Apr 1966
 - 
Mar 1969
Iwate Prefectural Morioka Daiichi High School
 
Apr 1969
 - 
Mar 1973
Biological Institute, Faculty of Science, Hirosaki University
 
Apr 1973
 - 
Mar 1975
Biological Institute, Master Course, Graduate School of Science, Tohoku University
 
 
 - 
1978
Graduate School, Division of Natural Science, Tohoku University
 
Apr 1975
 - 
Mar 1978
Biological Institute, Doctor Course, Graduate School of Science, Tohoku University
 

Committee Memberships

 
2009
 - 
2011
Japan Society of Protozoology  President
 

Awards & Honors

 
1996
The Award of the Japan Society of Protozoology
 
Oct 2010
Cosmopolitan Sophomore Nobel Prize'10
Winner: Fujishima, M.
 

Published Papers

 
Gaku Tokuda, Aram Mikaelyan, Chiho Fukui, Yu Matsuura, Hirofumi Watanabe, Masahiro Fujishima, Andreas Brune
Proceedings of the National Academy of Sciences of the United States of America.      Nov 2018   [Refereed]
Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae) is a two-step process that involves endogenous host cellulases secreted in the midgut and a dense bacterial community in the hindgut compartment. The genomes...
Identification of novel Legionella genes required for endosymbiosis in Paramecium based on comparative genome analysis with Holospora spp.
Watanabe K, Suzuki H, Nishida T, Mishima M, Tachibana M, Fujishima M, Shimizu T, Watarai M.
FEMS Microbiology Ecology      Aug 2018   [Refereed]
Takashi Nishida, Naho Hara, Kenta Watanabe, Takashi Shimizu, Masahiro Fujishima, Masahisa Watarai
Frontiers in Microbiology, section Microbial Symbioses   25    Apr 2018   [Refereed]
Claudia Vannini, Cristiana Sigona, Martin Hahn, Giulio Petroni, Masahiro Fujishima
European Journal of Protistology   59 124-132   May 2017   [Refereed]
in press   Aug 2014   [Refereed]
We present draft genome sequences of three Holospora species, hosted by the ciliate Paramecium caudatum; that is, the macronucleus-specific H. obtusa and&0a;the micronucleus-specific H. undulata and H. elegans. We investigate functions of ortholog...
15(183)    Mar 2014   [Refereed]
To expedite the process of gene discovery related to the endosymbiosis, we have undertaken Illumina deep sequencing of mRNAs prepared from symbiont-bearing and symbiont-free P. bursaria cells. We assembled the reads de novo to build the transcript...
Tonooka Y. and Fujishima M.
Applied Microbiology and Biotechnology   85(1) 37-43   2009
Mechanism for establishment of endosymbiosis between Paramecium bursaria and synbiotic Chlorella cells
Kodama, Y. and Fujishima, M.
Microscope   43(1) 60-63   2008
Abamo Fema, Dohra Hideo and Fujishima Masahiro
FEMS Microbiology Letters   280(1) 21-27   2008   [Refereed]
Kodama Yuuki and Fujishima Masahiro
Protist, 159 (3), 483-494, 2008   159(3) 483-494   2008   [Refereed]
Kodama Yuuki, Nakahara Miho and Fujishima Masahiro
Protoplasma   230(1-2) 61-67   2007   [Refereed]
Kodama Yuuki and Fujishima Masahiro
Protoplasma   231(1-2) 55-63   2007
Infectivity of Chlorella species for the ciliate Paramecium bursaria is not based on sugar residues of their cell wall components, but based on their ability to localize beneath the host cell membrane after escaping from the host digestive vacuole...
On the origin of mitochondria and Rickettsia-related eukaryotic endosymbionts. Review article.
B. Franz Lang, Henner Brinkman, Liisa Koski, Masahiro Fujishima Hans-Dieter Gortz and Gertraud Burger
Japanese Journal of Protozoology   38(2) 171-183   2005
Fokin Sergei I., Schweikert Michael and Fujishima Masahiro
Europ. J. Protistol.   41 129-138   2005   [Refereed]
Fujishima Masahiro, Kawai Miki and Yamamoto Ryu
FEMS Microbiology Letters   243(1) 101-105   2005   [Refereed]
Endonuclear symbiotic bacteria Holospora species distinguish the host nuclear envelopes.
Fujishima, M. and Kawai, M.
Endocytobiosis Cell Res.   15 71-76   2004   [Refereed]
Sergei I. Fokin, Ewa Przybos, Sergei M. Chivilev, Cora L. Beier, Matthias Horn, Bogumila Skotarczak, Beata Wodecka and Masahiro Fujishima
Europ. J. Protistol.   40(3) 225-243   2004   [Refereed]
Yoshimitsu Nakamura, Masako Aki, Tomonori Aikawa, Manabu Hori, Masahiro Fujishima.
FEMS Microbiology Letters   240(2) 209-213   2004   [Refereed]
Fokin, S. I., Schweikert, M., Gortz, H.-D. and Fujishima, M.
Europ. J. Protistol.   39(4) 475-480   2003   [Refereed]
Paramecium decaurelia and Paramecium dodecaurelia from the P. aurelia spp. complex in Japan.
Przybos, E., Fujishima, M., and Nakaoka, Y.
Folia biologica (Krakow)   51 223-224   2003   [Refereed]
Endonuclear symbiotic bacteria Holospora species that can distinguish of Paramecium species
Fujishima, M.
16(1) 35-47   1999
Interactions between the host Paramecium and its symbiont Holospora
Fujishima, M.
Japan Journal of Protozoology   30 1-13   1997
A macronuclear nucleoli-specific protein of the ciliate ┣DBParamecium(/)-┫DB ┣DBcaudatum(/)-┫DB
European Journal of Protistology   32(Suppl.I) 25-31   1996

Misc

 
In memoriam: Klaus Heckmann.
Masahiro Fujishima
Japan Journal of Protozoology   46(1, 2) 1-2   2013   [Invited]

Books etc

 
Biocommunication in ciliates (Eds, Guenther Witzany, Mariusz Nowacki)
Yuuki Kodama, Masahiro Fujishima (Part:Joint Work, Chapter 16. Paramecium as a model organism for studies on primary and secondary endosymbioses.)
Springer International Publishing Switzerland,   2016   ISBN:doi: 10.1007/978-3-319-32211-7_16  
Cilia/flagella and ciliates/flagellates, (Eds) Hausmann K., Radek R., Schweizerbart
Fujishima M., Kodama Y. (Part:Joint Work, Insights into the Paramecium-Holospora and Paramecium-Chlorella symbioses.)
Science Publishers, Stuttgart   Jan 2014   ISBN:978-3-510-65287-7
The Prokaryotes, 4th Edition, (Eds) Rosenberg E., DeLong E. F., Thompson F., Lory S., Stackebrand E., Quinones D.
Schweikert M., Fujishima M., Görtz H.-D. (Part:Joint Work, Symbiotic associations between ciliates and prokaryotes.)
Springer-Verlag Berlin Heidelberg   2013   ISBN:DOI 10.1007/978-3-642-30194-0_18
International Review of Cell and Molecular Biology", (Ed) Jeon K. W.
Kodama Y. and Fujishima M. (Part:Joint Work, Chapter 2. Secondary symbiosis between Paramecium and Chlorella cells.)
2010   ISBN:978-0-12-381011-3
Kodama Y. and Fujishima M. Chapter 2. Secondary symbiosis between Paramecium and Chlorella cells. In, International Review of Cell and Molecular Biology, K.W. Jeon (Ed.)
Academic Press   2010   
Endosymbionts in Paramecium
FUJISHIMA Masahiro (Part:Editor)
Springer   2009   
Kodama Y. and Fujishima M. Chapter 2. Infection of Paramecium bursaria with symbiotic Chlorella species. In, Endosymbionts in Paramecium. Microbiology Monographs Vol. 12, Fujishima M. (Ed.)
Springer   2009   ISBN:978-3-540-92676-4
Fujishima, M. Chapter 8. Infection and Maintenance of Holospora Species in Paramecium caudatum. In, Endosymbionts in Paramecium. Microbiology Monographs Vol. 12, Fujishima M. (Ed.).
Springer   2008   ISBN:978-3-540-92676-4
Fujishima M. and Kodama Y. Environmental adaptation of Paramecium by endosymbionts and application to remediation of ecosystem. In, Proceedings of Infrastructure and Environmental Management Symposium in Yamaguchi 2008, Miyamoto A. and Fujishima M. (・・・
Research Center for Environmental Safety (RCES), Yamaguchi University   2008   ISBN:9901161-9-4 C3060
Fujishima M. and Kodama Y. Environmental adaptation of Paramecium by endosymbionts and application to remediation of ecosystem. In, Proceedings of Infrastructure and Environmental Management Symposium in Yamaguchi 2008, Miyamoto A. and Fujishima...
Endosymbiotic bacteria and its genetics
Gentics of ┣DBParamecium(/)-┫DB. (Tohoku University Press)   1999   
┣DBParamecium(/)-┫DB
Spriuger Verlag   1988   

Conference Activities & Talks

 
Elucidation of the mechanism by which endsymbiosis is established using Paramecium [Invited]
FUJISHIMA Masahiro
363rd Workshop of Cell Engineering   22 Sep 2018   
Spirochaetes contribute to xylan degradation in the hindgut of wood-feeding higher termites [Invited]
Gaku Tokuda, Aram Mikaelyan, Chiho Fukui, Yu Matsuura, Hirofumi Watanabe, Masahiro Fujishima
17th International Symposium on Microbial Ecology   12 Aug 2018   
Prospects of Paramecium collection that has been used as a model organism for eukaryotic cells [Invited]
FUJISHIMA Masahiro
Workshop 7 “Introduction of the 4th NBRP collections as attractive research materials and the forefront of ABS information”, The 91th Annual Meeting of Japanese Society for Bacteriology   28 Mar 2018   
Legionella pneumonia TolC inhibits cellular trafficking in Paramecium tetraurelia
Nishida T, Hara N, Watanabe K, Shimizu T, Fujishima M, Watarai M.
27 Mar 2018   
Kodama, Y. and Fujishima, M. Infection process of symbiotic algae to the algae-free Paramecium bursaria.
XIII International Congress of Protistology   2009   
Masahiro Fujishima. Infection of Holospora and acquisition of stress resistance of the host Paramecium.
XIII International Congress of Protistology, Symposium “Symbiosis and Evolution”.   2009   
Kodama, Y. and Fujishima, M. Infection process of symbiotic algae to the algae-free Paramecium bursaria.
XIII International Congress of Protistology   2009   
Masahiro Fujishima. Infection of Holospora and acquisition of stress resistance of the host Paramecium.
XIII International Congress of Protistology, Symposium “Symbiosis and Evolution”.   2009   
Masahiro Fujishima. Infection and maintenance of Holospora in Paramecium.
International Symposium on Protistology. Evolution and Diversity   2008   
Masahiro Fujishima. Infection and maintenance of Holospora in Paramecium.
International Symposium on Protistology. Evolution and Diversity   2008   

Teaching Experience

 

Works

 
Joint Research on Mechanism of Infection and Maintenance of Endonuclear Symbiont Holospora
1995
Genomics and Proteomics of Endonuclear Symbiotic Bacterium Holospora
2000 - 2001
Joint Research on Mechanism of Infection and Maintenance of Endonuclear Symbiont Holospora
The Others   
Genomics and Proteomics of Endonuclear Symbiotic Bacterium Holospora
The Others   

Research Grants & Projects

 
Collection, preservation and provision of Paramewcium resources
AMED: National Bioresource Program, Core facility upgrading program
Project Year: Jun 2012 - Mar 2022    Investigator(s): FUJISHIMA Masahiro
Development of reliable cryopreservation method for Paramecium genus
AMED: National Bioresource Program, Fundamental technology upgrading program
Project Year: Oct 2017 - Mar 2019    Investigator(s): FUJISHIMA Masahiro
AZnalysis of a new defense mechanism against microbial infections found in Paramecium
JST: Grant-in-Aid for Challenging Exploratory Research
Project Year: Apr 2011 - Mar 2014    Investigator(s): FUJISHIMA Masahiro
Elucidation of establishment of endosymbiosis between Chrorella and Paramecium bursaria
JST: Grant-in-Aid for Scientific Research (B)
Project Year: Apr 2010 - Mar 2014    Investigator(s): FUJISHIMA Masahiro
Development of the world distribution map of the ciliate Paramecium and iuts endosymbiotic bacteria Holospora
JST: Grant-in-Aid for Scientific Research (B)
Project Year: Apr 2005 - Mar 2009    Investigator(s): FUJISHIMA Masahiro
JST: Grant-in-Aid for Scientific Research on Priority Area
Project Year: Apr 2002 - Mar 2003    Investigator(s): FUJISHIMA Masahiro
JST: Grant-in-Aid for Scientific Research on Priority Area (C)
Project Year: Apr 2001 - Mar 2002    Investigator(s): FUJISHIMA Masahiro
JST: Grant-in-Aid for Scientific Research on Priority Area (C)
Project Year: Apr 2000 - Mar 2001    Investigator(s): FUJISHIMA Masahiro
Interaction between endosymbniont Holospora and the host
JST: Grant-in-Aid for Scientific Research (B)
Project Year: Apr 1999 - Mar 2001    Investigator(s): FUJISHIMA Masahiro
Reexamination of Zoogeography in Southeast Asian by Protozoa
JST: Grant-in-Aid for Scientific Research (A)
Project Year: Apr 1997 - Mar 2000    Investigator(s): HOSHIDE Kazumi
JST: Grant-in-Aid for Encouragement of Young Scientists (A)
Project Year: Apr 1997 - Mar 1998    Investigator(s): FUJISHIMA Masahiro
JST: Grant-in-Aid for International Scientific Research
Project Year: Apr 1995 - Mar 1997    Investigator(s): FUJISHIMA Masahiro
Molecular Mechanism of infection and growth of endonuclear symbniotic bacterium
JST: Grant-in-Aid for General Scientific Research (B)
Project Year: Apr 1993 - Mar 1995    Investigator(s): FUJISHIMA Masahiro
Molecular and genetical research of developmental process in ciliates
JST: Grant-in-Aid for General Scientific Research (A)
Project Year: Apr 1991 - Mar 1994    Investigator(s): TAKAHASHI Mihiko
JST: Grant-in-Aid for General Scientific Research (C)
Project Year: Apr 1992 - Mar 1993    Investigator(s): FUJISHIMA Masahiro
JST: Grant-in-Aid for Co-operative Research (B)
Project Year: Apr 1991 - Mar 1993    Investigator(s): ISHIKAWA Hajime
Study of uclear membrane differentiation between germ and vegetative nuclei in early development of Paramecium
JST: Grant-in-Aid for General Scientific Research (C)
Project Year: Apr 1990 - Mar 1992    Investigator(s): FUJISHIMA Masahiro
JST: Grant-in-Aid for Encouragement of Young Scientists (A)
Project Year: Apr 1986 - Mar 1988    Investigator(s): FUJISHIMA Masahiro
JST: Grant-in-Aid for General Scientific Research (C)
Project Year: Apr 1984 - Mar 1986    Investigator(s): FUJISHIMA Masahiro
JST: Grant-in-Aid for Encouragement of Young Scientists (A)
Project Year: Apr 1983 - Mar 1984    Investigator(s): FUJISHIMA Masahiro
JST: Grant-in-Aid for Encouragement of Young Scientists (A)
Project Year: Apr 1982 - Mar 1983    Investigator(s): FUJISHIMA Masahiro
Control Mechanism of Establishment of Endosymbiosis