論文

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2022年5月30日

Endosymbiotic Chlorella variabilis reduces mitochondrial number in the ciliate Paramecium bursaria

Scientific Reports
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回数 : 39
  • Yuuki Kodama
  • ,
  • Masahiro Fujishima

12
1
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41598-022-12496-8
出版者・発行元
Springer Science and Business Media LLC

Abstract

Extant symbioses illustrate endosymbiosis is a driving force for evolution and diversification. In the ciliate Paramecium bursaria, the endosymbiotic alga Chlorella variabilis in perialgal vacuole localizes beneath the host cell cortex by adhesion between the perialgal vacuole membrane and host mitochondria. We investigated whether host mitochondria are also affected by algal endosymbiosis. Transmission electron microscopy of host cells showed fewer mitochondria beneath the algae-bearing host cell cortex than that of alga-free cells. To compare the density and distribution of host mitochondria with or without symbiotic algae, we developed a monoclonal antibody against Paramecium mitochondria. Immunofluorescence microscopy with the monoclonal antibody showed that the mitochondrial density of the algae-bearing P. bursaria was significantly lower than that of the alga-free cells. The total cell protein concentration of alga-free P. bursaria cells was approximately 1.8-fold higher than that of algae-bearing cells, and the protein content of mitochondria was significantly higher in alga-free cells than that in the algae-bearing cells. These results corresponded with those obtained by transmission electron and immunofluorescence microscopies. This paper shows that endosymbiotic algae affect reduced mitochondrial number in the host P. bursaria significantly.

リンク情報
DOI
https://doi.org/10.1038/s41598-022-12496-8 本文へのリンクあり
URL
https://www.nature.com/articles/s41598-022-12496-8.pdf
URL
https://www.nature.com/articles/s41598-022-12496-8
ID情報
  • DOI : 10.1038/s41598-022-12496-8
  • eISSN : 2045-2322

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