論文

査読有り 筆頭著者
2013年7月

Age- and sex-dependent DNA hypomethylation controlled by growth hormone in mouse liver

MECHANISMS OF AGEING AND DEVELOPMENT
  • Masaki Takasugi
  • ,
  • Koji Hayakawa
  • ,
  • Daisuke Arai
  • ,
  • Kunio Shiota

134
7-8
開始ページ
331
終了ページ
337
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.mad.2013.05.003
出版者・発行元
ELSEVIER IRELAND LTD

In mammals, differences in liver function and aging have been observed between sexes; however, the epigenetic mechanisms underlying such differences remain largely unexplored. In this study, we investigated sex- and age-dependent DNA methylation status in the mouse liver. We analyzed 90 known sex-differentially expressed genes, and identified sex-dependent methylation in Zfp809, Hsd3b5, Treh, Cxcl11, Cyp17a1, and Nnmt genes. After 4 weeks of age, we noted the gradual establishment of sex-dependent hypomethylation in each of these genes in either males or females. The exposure of male mice to female-like growth hormone (GH) profile repressed male-predominant hypomethylation and promoted female-predominant hypomethylation. The occurrence of age-dependent hypomethylation, including at loci for which we also observed sex-dependent changes in DNA methylation, was accompanied by the downregulation of DNMT3A/B. In addition, we found that age-dependent hypomethylation was promoted through liver regeneration induced by partial hepatectomy, suggesting that DNMT activities were not enough to retain methylation levels. In conclusion, our results demonstrate that sex-dependent GH profiles influence the age-progressive hypomethylation under decreased DNMT3A/B levels in certain regions of the genome. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

Web of Science ® 被引用回数 : 16

リンク情報
DOI
https://doi.org/10.1016/j.mad.2013.05.003
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000322054700006&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.mad.2013.05.003
  • ISSN : 0047-6374
  • Web of Science ID : WOS:000322054700006

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