論文

査読有り
2014年4月

Quantitative Analysis of Coenzyme F430 in Environmental Samples: A New Diagnostic Tool for Methanogenesis and Anaerobic Methane Oxidation

ANALYTICAL CHEMISTRY
  • Masanori Kaneko
  • ,
  • Yoshinori Takano
  • ,
  • Yoshito Chikaraishi
  • ,
  • Nanako O. Ogawa
  • ,
  • Susumu Asakawa
  • ,
  • Takeshi Watanabe
  • ,
  • Seigo Shima
  • ,
  • Martin Krueger
  • ,
  • Makoto Matsushita
  • ,
  • Hiroyuki Kimura
  • ,
  • Naohiko Ohkouchi

86
7
開始ページ
3633
終了ページ
3638
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1021/ac500305j
出版者・発行元
AMER CHEMICAL SOC

Coenzyme F430 is a nickel hydrocorphinoid and is the prosthetic group of methyl coenzyme M reductase that catalyzes the last step of the methanogenic reaction sequence and its reversed reaction for anaerobic methane oxidation by ANME. As such, function-specific compound analysis has the potential to reveal the microbial distribution and activity associated with methane production and consumption in natural environments and, in particular, in deep subsurface sediments where microbiological and geochemical techniques are restricted. Herein, we report the development of a technique for high-sensitivity analysis of F430 in environmental samples, including paddy soils, marine sediments, microbial mats, and an anaerobic groundwater. The lower detection limit of F430 analysis by liquid chromatography/mass spectrometry is 0.1 femto mol, which corresponds to 6 x 10(2) to 1 x 10(4) cells of methanogens. F430 concentrations in these natural environmental samples range from 63 X 10(-6) to 44 nmol g(-1) and are consistent with the methanogenic archaeal biomass estimated by microbiological analyses.

Web of Science ® 被引用回数 : 16

リンク情報
DOI
https://doi.org/10.1021/ac500305j
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000333776600052&DestApp=WOS_CPL
URL
http://www.scopus.com/inward/record.url?eid=2-s2.0-84897499401&partnerID=MN8TOARS
URL
http://orcid.org/0000-0003-0787-4731

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