2014年
On-chip self-assembly of cell embedded microstructures to vascular-like microtubes
LAB ON A CHIP
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- 巻
- 14
- 号
- 6
- 開始ページ
- 1151
- 終了ページ
- 1161
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1039/c3lc51134k
- 出版者・発行元
- ROYAL SOC CHEMISTRY
Currently, research on the construction of vascular-like tubular structures is a hot area of tissue engineering, since it has potential applications in the building of artificial blood vessels. In this paper, we report a fluidic self-assembly method using cell embedded microstructures to construct vascular-like microtubes. A novel 4-layer microfluidic device was fabricated using polydimethylsiloxane (PDMS), which contains fabrication, self-assembly and extraction areas inside one channel. Cell embedded microstructures were directly fabricated using poly(ethylene glycol) diacrylate (PEGDA) in the fabrication area, namely on-chip fabrication. Self-assembly of the fabricated microstructures was performed in the assembly area which has a micro well. Assembled tubular structures (microtubes) were extracted outside the channel into culture dishes using a normally closed (NC) micro valve in the extraction area. The self-assembly mechanism was experimentally demonstrated. The performance of the NC micro valve and embedded cell concentration were both evaluated. Fibroblast (NIH/3T3) embedded vascular-like microtubes were constructed inside this reusable microfluidic device.
- リンク情報
- ID情報
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- DOI : 10.1039/c3lc51134k
- ISSN : 1473-0197
- eISSN : 1473-0189
- Web of Science ID : WOS:000331804900013