論文

国際誌
2022年9月12日

Fluorochromized tyramide-glucose oxidase as a multiplex fluorescent tyramide signal amplification system for histochemical analysis.

Scientific reports
  • Kenta Yamauchi
  • Shinichiro Okamoto
  • Yoko Ishida
  • Kohtarou Konno
  • Kisara Hoshino
  • Takahiro Furuta
  • Megumu Takahashi
  • Masato Koike
  • Kaoru Isa
  • Masahiko Watanabe
  • Tadashi Isa
  • Hiroyuki Hioki
  • 全て表示

12
1
開始ページ
14807
終了ページ
14807
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41598-022-19085-9

Tyramide signal amplification (TSA) is a highly sensitive method for histochemical analysis. Previously, we reported a TSA system, biotinyl tyramine-glucose oxidase (BT-GO), for bright-filed imaging. Here, we develop fluorochromized tyramide-glucose oxidase (FT-GO) as a multiplex fluorescent TSA system. FT-GO involves peroxidase-catalyzed deposition of fluorochromized tyramide (FT) with hydrogen peroxide produced by enzymatic reaction between glucose and glucose oxidase. We showed that FT-GO enhanced immunofluorescence signals while maintaining low background signals. Compared with indirect immunofluorescence detections, FT-GO demonstrated a more widespread distribution of monoaminergic projection systems in mouse and marmoset brains. For multiplex labeling with FT-GO, we quenched antibody-conjugated peroxidase using sodium azide. We applied FT-GO to multiplex fluorescent in situ hybridization, and succeeded in labeling neocortical interneuron subtypes by coupling with immunofluorescence. FT-GO immunofluorescence further increased the detectability of an adeno-associated virus tracer. Given its simplicity and a staining with a high signal-to-noise ratio, FT-GO would provide a versatile platform for histochemical analysis.

リンク情報
DOI
https://doi.org/10.1038/s41598-022-19085-9
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/36097273
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9468149
ID情報
  • DOI : 10.1038/s41598-022-19085-9
  • PubMed ID : 36097273
  • PubMed Central 記事ID : PMC9468149

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