2007年6月
Regulation of dendritogenesis via a lipid-raft-associated Ca2+/calmodulin-dependent protein kinase CLICK-III/CaMKl gamma
NEURON
- 巻
- 54
- 号
- 5
- 開始ページ
- 755
- 終了ページ
- 770
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.neuron.2007.05.021
- 出版者・発行元
- CELL PRESS
Ca2+ signaling plays a central role in activity-dependent regulation of dendritic arborization, but key molecular mechanisms downstream of calcium elevation remain poorly understood. Here we show that the C-terminal region of the Ca2+/calmodulin-dependent protein kinase CLICK-III (CL3)/CaMKl gamma, a membrane-anchored CaMK, was uniquely modified by two sequential lipidification steps: prenylation followed by a kinase-activity-regulated palmitoylation. These modifications were essential for CL3 membrane anchoring and targeting into detergent-resistant lipid microdomains (or rafts) in the dendrites. We found that CL3 critically contributed to BDNF-stimulated dendritic growth. Raft insertion of CL3 specifically promoted dendritogenesis of cortical neurons by acting upstream of RacGEF STEF and Rac, both present in lipid rafts. Thus, CL3 may represent a key element in the Ca2+-dependent and lipid-raft-delineated switch that turns on extrinsic activity-regulated dendrite formation in developing cortical neurons.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.neuron.2007.05.021
- ISSN : 0896-6273
- eISSN : 1097-4199
- Web of Science ID : WOS:000247329900009