2017年10月
Production of highly immunogenic virus-like particles of bovine papillomavirus type 6 in silkworm pupae
VACCINE
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- 巻
- 35
- 号
- 43
- 開始ページ
- 5878
- 終了ページ
- 5882
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.vaccine.2017.08.079
- 出版者・発行元
- ELSEVIER SCI LTD
Bovine papillomaviruses (BPVs) are the causative agent of bovine teat papillomatosis, which can lead to severe economic losses in dairy cattle. Among the 14 identified BPV genotypes, BPV type 6 (BPV6) is the most frequently detected in teat papilloma lesions, and is therefore thought to play a major role in teat papillomatosis. To develop an effective vaccine against BPV6 infection, we produced virus-like particles of BPV6 (BPV6-VLP) in silkworm (Bombyx mori) pupae and purified these by heparin affinity chromatography using a single column. About 0.7 mg purified BPV6-VLP was obtained from one pupa. BPV6-VLPimmunized mice produced a specific IgG to BPV6 that recognized BPV6 antigen with high sensitivity in an immunohistochemical analysis. Thus, silkworm pupae are a useful bioreactor for the production of BPV6-VLP, which can potentially be used as a vaccine for bovine teat papillomatosis. (C) 2017 Elsevier Ltd. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.vaccine.2017.08.079
- ISSN : 0264-410X
- eISSN : 1873-2518
- PubMed ID : 28893480
- Web of Science ID : WOS:000413057700024