論文

査読有り 国際誌
2020年7月30日

Ligation of MHC Class II Induces PKC-Dependent Clathrin-Mediated Endocytosis of MHC Class II.

Cells
  • Kento Masaki
  • ,
  • Yuhji Hiraki
  • ,
  • Hiroka Onishi
  • ,
  • Yuka Satoh
  • ,
  • Paul A Roche
  • ,
  • Satoshi Tanaka
  • ,
  • Kazuyuki Furuta

9
8
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3390/cells9081810

In addition to antigen presentation to CD4+ T cells, aggregation of cell surface major histocompatibility complex class II (MHC-II) molecules induces signal transduction in antigen presenting cells that regulate cellular functions. We previously reported that crosslinking of MHC-II induced the endocytosis of MHC-II, which was associated with decreased surface expression levels in murine dendritic cells (DCs) and resulted in impaired activation of CD4+ T cells. However, the downstream signal that induces MHC-II endocytosis remains to be elucidated. In this study, we found that the crosslinking of MHC-II induced intracellular Ca2+ mobilization, which was necessary for crosslinking-induced MHC-II endocytosis. We also found that these events were suppressed by inhibitors of Syk and phospholipase C (PLC). Treatments with a phorbol ester promoted MHC-II endocytosis, whereas inhibitors of protein kinase C (PKC) suppressed crosslinking-induced endocytosis of MHC-II. These results suggest that PKC could be involved in this process. Furthermore, crosslinking-induced MHC-II endocytosis was suppressed by inhibitors of clathrin-dependent endocytosis. Our results indicate that the crosslinking of MHC-II could stimulate Ca2+ mobilization and induce the clathrin-dependent endocytosis of MHC-II in murine DCs.

リンク情報
DOI
https://doi.org/10.3390/cells9081810
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32751549
ID情報
  • DOI : 10.3390/cells9081810
  • PubMed ID : 32751549

エクスポート
BibTeX RIS