2016年
Liposome chaperon in cell-free membrane protein synthesis: one-step preparation of KcsA-integrated liposomes and electrophysiological analysis by the planar bilayer method
BIOMATERIALS SCIENCE
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- 巻
- 4
- 号
- 2
- 開始ページ
- 258
- 終了ページ
- 264
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1039/c5bm00285k
- 出版者・発行元
- ROYAL SOC CHEMISTRY
Chaperoning functions of liposomes were investigated using cell-free membrane protein synthesis. KcsA potassium channel-reconstituted liposomes were prepared directly using cell-free protein synthesis. In the absence of liposomes, all synthesized KcsA protein aggregated. In the presence of liposomes, however, synthesized KcsA spontaneously integrated into the liposome membrane. The KscA-reconstituted liposomes were transferred to the planar bilayer across a small hole in a thin plastic sheet and the channel function of KcsA was examined. The original electrophysiological activities, such as voltage-and pH-dependence, were observed. These results suggested that in cell-free membrane protein synthesis, liposomes act as chaperones, preventing aggregation and assisting in folding and tetrameric formation, thereby allowing full channel activity.
- リンク情報
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- DOI
- https://doi.org/10.1039/c5bm00285k
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/26548774
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000368945400007&DestApp=WOS_CPL
- URL
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84955590468&origin=inward
- ID情報
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- DOI : 10.1039/c5bm00285k
- ISSN : 2047-4830
- eISSN : 2047-4849
- PubMed ID : 26548774
- SCOPUS ID : 84955590468
- Web of Science ID : WOS:000368945400007