We designed the fusogenic membrane protein-expressing plasmid DNAs to achieve the development of newly encapsulation methods and drug delivery systems based on membrane proteins reconstituted liposomes, proteoliposome. The cell-free fusogenic membrane protein synthesis was performed in the presence of liposomes. The fusogenic membrane proteins were successfully integrated into lipsosomal membrane, and we then evaluated the their fusion activities. Unfortunately, we failed to obtain the fusogenic proteoliposome in the present study. These findings indicated that it is necessary to add other molecular chaperons or regulate the post-translational modification to form the correct conformation of fusogenic membrane proteins in the present system.