論文

査読有り 国際誌
2019年2月12日

Cancer-Specifically Re-Spliced TSG101 mRNA Promotes Invasion and Metastasis of Nasopharyngeal Carcinoma.

International journal of molecular sciences
  • Huey-Huey Chua
  • ,
  • Toshiki Kameyama
  • ,
  • Akila Mayeda
  • ,
  • Te-Huei Yeh

20
3
開始ページ
773
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3390/ijms20030773

TSG101 (Tumor susceptibility 101) gene and its aberrantly spliced isoform, termed TSG101∆154-1054, are tightly linked to tumorigenesis in various cancers. The aberrant TSG101∆154-1054 mRNA is generated from cancer-specific re-splicing of mature TSG101 mRNA. The TSG101∆154-1054 protein protects the full-length TSG101 protein from ubiquitin-mediated degradation, implicating TSG101∆154-1054 protein in the progression of cancer. Here, we confirmed that the presence of TSG101∆154-1054 mRNA indeed caused an accumulation of the TSG101 protein in biopsies of human nasopharyngeal carcinoma (NPC), which was recapitulated by the overexpression of TSG101∆154-1054 in the NPC cell line TW01. We demonstrate the potential function of the TSG101∆154-1054 protein in the malignancy of human NPC with scratch-wound healing and transwell invasion assays. By increasing the stability of the TSG101 protein, TSG101∆154-1054 specifically enhanced TSG101-mediated TW01 cell migration and invasion, suggesting the involvement in NPC metastasis in vivo. This finding sheds light on the functional significance of TSG101∆154-1054 generation via re-splicing of TSG101 mRNA in NPC metastasis and hints at its potential importance as a therapeutic target.

リンク情報
DOI
https://doi.org/10.3390/ijms20030773
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30759747
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387056
ID情報
  • DOI : 10.3390/ijms20030773
  • PubMed ID : 30759747
  • PubMed Central 記事ID : PMC6387056

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