1998年
Oxidative stress suppresses the endothelial secretion of endothelin
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
- 巻
- 31
- 号
- 開始ページ
- S345
- 終了ページ
- S347
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1097/00005344-199800001-00096
- 出版者・発行元
- LIPPINCOTT WILLIAMS & WILKINS
To address endothelial function on vascular walls exposed to oxidative stress, we investigated the effect of oxidative stress on the secretion of endothelin-1 (ET-1) from cultured bovine carotid artery endothelial cells (BAECs). Concentrations of ET-1 in the media were measured by a specific radioimmunoassay and ET-1 mRNA expression was estimated by Northern blot analysis. Treatment of BAECs with 0.5-2.0 mM H2O2 for 3 h suppressed both ET-1 secretion and ET-1 mRNA expression in a dose-dependent manner compared to control. Attenuation of ET-1 mRNA expression by H2O2 was revealed to take place at the transcriptional level. The addition of N-G-nitro-L-arginine-methyl ester (L-NAME) 10 mum, a specific nitric oxide synthase inhibitor, had no effect on H2O2-induced suppression of ET-1 mRNA expression. Suppression of ET secretion under oxidative stress observed in the present study is proposed to be a compensatory mechanism of endothelial cells to inhibit vasoconstriction and proliferation during oxidative stress.
- リンク情報
- ID情報
-
- DOI : 10.1097/00005344-199800001-00096
- ISSN : 0160-2446
- PubMed ID : 9595477
- Web of Science ID : WOS:000174051600096