論文

査読有り
2018年1月1日

Oxidative Stress in the Visceral Fat Is Elevated in Postmenopausal Women with Gynecologic Cancer

Journal of Women's Health
  • Megumi Narumi
  • ,
  • Kazuhiro Takahashi
  • ,
  • Hizuru Yamatani
  • ,
  • Manabu Seino
  • ,
  • Keiko Yamanouchi
  • ,
  • Tsuyoshi Ohta
  • ,
  • Toshifumi Takahashi
  • ,
  • Hirohisa Kurachi
  • ,
  • Satoru Nagase

27
1
開始ページ
99
終了ページ
106
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1089/jwh.2016.6301
出版者・発行元
Mary Ann Liebert Inc.

Objectives: This study aimed to evaluate differences in oxidative stress of visceral fat between premenopausal and postmenopausal women and clarify the antioxidant effect of estrogen on adipocytes. Materials and Methods: Abdominal subcutaneous and omental visceral adipose tissues were obtained from 38 patients who underwent gynecological surgery. We measured the sizes of the adipocytes and evaluated the lipid peroxidation levels in the adipose tissues. We investigated whether estrogen inhibited the intracellular reactive oxygen species (ROS) production that was induced by hydrogen peroxide (H2O2) in 3T3-L1 adipocytes. Results: The visceral adipocytes were 1162.4 μm2 and 1881.9 μm2 in premenopausal and postmenopausal women, respectively
hence they were significantly larger in the latter (p &lt
0.05). The lipid peroxidation levels were 46.7 nmoL/mg protein in premenopausal women and 99.6 nmoL/mg protein in postmenopausal women
hence the lipid peroxidation levels were significantly higher in the latter (p &lt
0.05). Estradiol (E2) significantly reduced the intracellular ROS levels that were induced by H2O2 in 3T3-L1 adipocytes (p &lt
0.01). We determined that E2 significantly increased the expression of nuclear factor erythroid 2-related factor 2 (NRF2)-dependent antioxidant genes, heme oxygenase-1 (HO-1), NAD(P)H:quinone oxidoreductase 1 (NQO1), and the glutamate-cysteine ligase (GCL) modifier subunit genes, in 3T3-L1 adipocytes (p &lt
0.01). Conclusion: Oxidative stress in the visceral fat is higher in postmenopausal women. The expression of the antioxidant genes HO-1, NQO1, and GCL was upregulated by estrogen in 3T3-L1 adipocytes. Hence, estrogen may act as an antioxidant in the adipose tissues of premenopausal women.

リンク情報
DOI
https://doi.org/10.1089/jwh.2016.6301
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28816586
ID情報
  • DOI : 10.1089/jwh.2016.6301
  • ISSN : 1931-843X
  • ISSN : 1540-9996
  • PubMed ID : 28816586
  • SCOPUS ID : 85040826747

エクスポート
BibTeX RIS