論文

査読有り 国際誌
2019年1月11日

Metaepigenomic analysis reveals the unexplored diversity of DNA methylation in an environmental prokaryotic community.

Nature communications
  • Satoshi Hiraoka
  • ,
  • Yusuke Okazaki
  • ,
  • Mizue Anda
  • ,
  • Atsushi Toyoda
  • ,
  • Shin-Ichi Nakano
  • ,
  • Wataru Iwasaki

10
1
開始ページ
159
終了ページ
159
記述言語
英語
掲載種別
DOI
10.1038/s41467-018-08103-y

DNA methylation plays important roles in prokaryotes, and their genomic landscapes-prokaryotic epigenomes-have recently begun to be disclosed. However, our knowledge of prokaryotic methylation systems is focused on those of culturable microbes, which are rare in nature. Here, we used single-molecule real-time and circular consensus sequencing techniques to reveal the 'metaepigenomes' of a microbial community in the largest lake in Japan, Lake Biwa. We reconstructed 19 draft genomes from diverse bacterial and archaeal groups, most of which are yet to be cultured. The analysis of DNA chemical modifications in those genomes revealed 22 methylated motifs, nine of which were novel. We identified methyltransferase genes likely responsible for methylation of the novel motifs, and confirmed the catalytic specificities of four of them via transformation experiments using synthetic genes. Our study highlights metaepigenomics as a powerful approach for identification of the vast unexplored variety of prokaryotic DNA methylation systems in nature.

リンク情報
DOI
https://doi.org/10.1038/s41467-018-08103-y
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30635580
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6329791
URL
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=MEDLINE&KeyUT=MEDLINE:30635580&KeyUID=MEDLINE:30635580
URL
http://orcid.org/0000-0002-9169-9245

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