論文

査読有り
2016年6月

Generation of Transparent Zebrafish with Fluorescent Ovaries: A Living Visible Model for Reproductive Biology

ZEBRAFISH
  • Afroza Akhter
  • ,
  • Ryo-ichi Kumagai
  • ,
  • Shimi Rani Roy
  • ,
  • Sanae Ii
  • ,
  • Mika Tokumoto
  • ,
  • Babul Hossain
  • ,
  • Jun Wang
  • ,
  • Wanlada Klangnurak
  • ,
  • Takehiro Miyazaki
  • ,
  • Toshinobu Tokumoto

13
3
開始ページ
155
終了ページ
160
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1089/zeb.2015.1116
出版者・発行元
MARY ANN LIEBERT, INC

The transparent zebrafish enables researchers to study the morphology and distribution of cells and tissues in vivo. To capture the dynamic processes of germ cell proliferation and juvenile ovarian development in zebrafish in vivo, we established transgenic (TG) lines to allow us to monitor the changes in the ovaries of living fish. The original transgenic line with ovarian fluorescence was occasionally established. Although the cDNA integrated in the strain was constructed for the expression of enhanced green fluorescent protein (EGFP) driven by the medaka beta-actin promoter, expression of EGFP is restricted to the oocytes and gills in adult fish. Mutant strains with transparent bodies, roy and ruby, were isolated in zebrafish. In this study, we crossed the TG strain with fluorescent ovary with transparent strains and established the TG (beta-actin: EGFP); ruby strain. The strain is highly transparent, and the oocytes are easily observed in living fish. We identified a fluorescent tissue that might contain the undifferentiated germ cells close to the cloaca in the strain. This strain can be used for analysis of ovarian development in vivo.

Web of Science ® 被引用回数 : 8

リンク情報
DOI
https://doi.org/10.1089/zeb.2015.1116
Scopus
https://www.scopus.com/record/display.uri?eid=2-s2.0-84971554622&origin=inward
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000377213000001&DestApp=WOS_CPL
URL
http://www.scopus.com/inward/record.url?eid=2-s2.0-84971554622&partnerID=MN8TOARS
URL
http://orcid.org/0000-0002-6638-3986