2008年6月
A novel method for RNA interference in neurons using enhanced green fluorescent protein (EGFP)-transgenic rats
NEUROSCIENCE RESEARCH
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- ,
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- 巻
- 61
- 号
- 2
- 開始ページ
- 219
- 終了ページ
- 224
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.neures.2008.02.008
- 出版者・発行元
- ELSEVIER IRELAND LTD
RNA interference (RNAi) is the simplest way of examining gene function by inhibiting expression. However, due to the low rate of introducing short interfering RNA (siRNA) into neurons, it is difficult to discriminate into which neurons that have been successfully introduced. Here, we used neurons from transgenic rats expressing enhanced green fluorescent protein (EGFP), and we simultaneously applied small interfering RNAs (siRNAs) against EGFP and a targeted gene to the EGFP-expressing neurons. EGFP fluorescence and immunoreactivity of the protein were then assessed by immunofluorescence microscopy. Quantitative analysis of the immunofluorescence confirmed that loss of EGFP closely correlates with loss of the target protein. These results indicate that this method can be used in a wider range of the neuroscientific research, especially in genome-wide studies. (c) 2008 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.neures.2008.02.008
- CiNii Articles
- http://ci.nii.ac.jp/naid/80019603762
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/18423674
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000256508000010&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1016/j.neures.2008.02.008
- ISSN : 0168-0102
- CiNii Articles ID : 80019603762
- PubMed ID : 18423674
- Web of Science ID : WOS:000256508000010