2010年8月
Neuroglycan C, A Brain-Specific Chondroitin Sulfate Proteoglycan, Interacts with Pleiotrophin, A Heparin-Binding Growth Factor
NEUROCHEMICAL RESEARCH
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- 巻
- 35
- 号
- 8
- 開始ページ
- 1131
- 終了ページ
- 1137
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s11064-010-0164-9
- 出版者・発行元
- SPRINGER/PLENUM PUBLISHERS
Neuroglycan C (NGC) is a transmembrane-type chondroitin sulfate proteoglycan that promotes neurite outgrowth. To identify the ligand of NGC, we applied a detergent-solubilized membrane fraction of fetal rat brains to an NGC-immobilized affinity column. Several proteins were eluted from the column including an 18 kDa-band protein recognized by an anti-pleiotrophin antibody. The binding of pleiotrophin (PTN) to NGC was confirmed by a quartz crystal microbalance method and had a Kd of 8.7 nM. PTN bound to the acidic amino acid cluster of the NGC extracellular domain. In addition, PTN bound to both chondroitin sulfate-bearing NGC and chondroitinase-treated NGC prepared from the neonatal rat brain. These results suggest that NGC interacts with PTN.
- リンク情報
- ID情報
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- DOI : 10.1007/s11064-010-0164-9
- ISSN : 0364-3190
- eISSN : 1573-6903
- PubMed ID : 20369290
- Web of Science ID : WOS:000279226300002