2013年9月
Molecular cloning and tumour suppressor function analysis of canine REIC/Dkk-3 in mammary gland tumours
VETERINARY JOURNAL
- 巻
- 197
- 号
- 3
- 開始ページ
- 769
- 終了ページ
- 775
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.tvjl.2013.04.024
- 出版者・発行元
- ELSEVIER SCI LTD
REIC/Dkk-3, a member of the human Dickkopf (Dkk) family, plays a role as a suppressor of growth in several human cancers. In this study, the tumour suppression function of canine REIC/Dkk-3 was investigated. The full-length open reading frame of the canine REIC/Dkk-3 homologue was cloned and the tissue distribution of REIC/Dkk-3 mRNA was determined, along with the subcellular localisation of the REIC/Dkk-3 protein in canine cancer cell lines. Expression of REIC/Dkk-3 was lower in mammary gland tumours and in canine mammary carcinoma cell lines than in normal mammary gland tissue. Overexpression of REIC/Dkk-3 induced apoptosis in canine mammary carcinoma cell lines. These results show that expression of REIC/Dkk-3 is downregulated in canine mammary tumours and that one of the functions of this gene is induction of apoptosis. (C) 2013 Elsevier Ltd. All rights reserved.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.tvjl.2013.04.024
- ISSN : 1090-0233
- eISSN : 1532-2971
- PubMed ID : 23732075
- Web of Science ID : WOS:000326667600043