論文

査読有り
2019年9月3日

Metabolite profiling in sphere-forming cells from canine mammary adenocarcinoma cell lines using gas chromatography-mass spectrometry.

The Journal of veterinary medical science
  • Masaki Michishita
  • ,
  • Namika Saito
  • ,
  • Satoshi Nozawa
  • ,
  • Rina Furumoto
  • ,
  • Takayuki Nakagawa
  • ,
  • Touko Sato
  • ,
  • Kazuhiko Ochiai
  • ,
  • Daigo Azakami
  • ,
  • Kinya Katayama
  • ,
  • Rei Nakahira
  • ,
  • Hiroyuki Tazaki
  • ,
  • Yukino Machida
  • ,
  • Toshiyuki Ishiwata

81
9
開始ページ
1238
終了ページ
1248
記述言語
英語
掲載種別
DOI
10.1292/jvms.19-0194

Cancer consists of heterogeneous cells that contain a small population of cells that possess stem cell properties; these cells, referred to as cancer stem cells (CSCs) or tumor-initiating cells, are involved in tumor progression and metastasis. Using a sphere-forming assay, canine mammary CSCs were found to be similar to human breast CSCs. Metabolic reprogramming has been recognized as a hallmark of various cancers. However, the significance of cellular metabolism in CSCs remains unclear. The aim of this study was to define the metabolic characteristics of CSCs derived from canine mammary tumors and gain an understanding of the maintenance of stemness. We identified metabolite profiles of canine mammary adenocarcinoma cell lines using gas chromatography-mass spectrometry. Metabolites were extracted from both adherent and sphere-forming cells derived from three cell lines. Sphere-forming cells were separated from adherent cells using an orthogonal, partial least-squares discriminant analysis. Sphere-forming cells were found to contain high levels of the amino acids alanine, glycine and proline compared with adherent cells. They also had high levels of palmitoleate, palmitate and dihomo-gamma-linolenic acid compared with adherent cells. In a sphere-forming assay, palmitate increased the number of spheres for all cell lines. These results indicate that the sphere-forming cells derived from canine mammary adenocarcinoma cell lines have specific metabolic profiles that may be useful for the development of CSC-specific therapies targeting metabolic pathways and potential stemness biomarkers; these results also clarify the maintenance of stemness in canine mammary CSCs.

リンク情報
DOI
https://doi.org/10.1292/jvms.19-0194
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31308293
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6785626