2011年
Quantification of pork, chicken and beef by using a novel reference molecule.
Bioscience, biotechnology, and biochemistry
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- 巻
- 75
- 号
- 9
- 開始ページ
- 1639
- 終了ページ
- 43
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
A standard plasmid was constructed as a novel reference molecule for use in real-time quantitative PCR assays to verify the identity of beef, pork, chicken, mutton, and horseflesh. The plasmid contained a target domain of the cytochrome b (cyt b) gene and an artificial DNA sequence. Primers CO-F and CO-R, and probe CO-P were specifically designed to detect the artificial sequence. The calculated R² values of the standard curves (10³-10⁷ copies per reaction) for the five species ranged between 0.998 and 0.999 in the quantification analysis. The constructed plasmid provides a universal method for measuring the copy number of cyt b DNA in minced meat. This method would be a useful procedure for verifying food labels.
- リンク情報
- ID情報
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- eISSN : 1347-6947
- PubMed ID : 21897035