論文

査読有り 最終著者 責任著者 国際誌
2018年4月

X‐ray‐enhanced cancer cell migration requires the linker of nucleoskeleton and cytoskeleton complex

Cancer Science
  • Hiromasa Imaizumi
  • ,
  • Katsutoshi Sato
  • ,
  • Asuka Nishihara
  • ,
  • Kazumasa Minami
  • ,
  • Masahiko Koizumi
  • ,
  • Nariaki Matsuura
  • ,
  • Miki Hieda

109
4
開始ページ
1158
終了ページ
1165
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/cas.13545
出版者・発行元
Wiley

The linker of nucleoskeleton and cytoskeleton (LINC) complex is a multifunctional protein complex that is involved in various processes at the nuclear envelope, including nuclear migration, mechanotransduction, chromatin tethering and DNA damage response. We recently showed that a nuclear envelope protein, Sad1 and UNC84 domain protein 1 (SUN1), a component of the LINC complex, has a critical function in cell migration. Although ionizing radiation activates cell migration and invasion in vivo and in vitro, the underlying molecular mechanism remains unknown. Here, we examined the involvement of the LINC complex in radiation-enhanced cell migration and invasion. A sublethal dose of X-ray radiation promoted human breast cancer MDA-MB-231 cell migration and invasion, whereas carbon ion beam radiation suppressed these processes in a dose-dependent manner. Depletion of SUN1 and SUN2 significantly suppressed X-ray-enhanced cell migration and invasion. Moreover, depletion or overexpression of each SUN1 splicing variant revealed that SUN1_888 containing 888 amino acids of SUN1 but not SUN1_916 was required for X-ray-enhanced migration and invasion. In addition, the results suggested that X-ray irradiation affected the expression level of SUN1 splicing variants and a SUN protein binding partner, nesprins. Taken together, our findings supported that the LINC complex contributed to photon-enhanced cell migration and invasion.

リンク情報
DOI
https://doi.org/10.1111/cas.13545
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29465769
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5891189
ID情報
  • DOI : 10.1111/cas.13545
  • ORCIDのPut Code : 43425146
  • PubMed ID : 29465769
  • PubMed Central 記事ID : PMC5891189

エクスポート
BibTeX RIS