論文

査読有り 国際誌
2018年

Live Imaging of Fetal Intra-abdominal Organs Using Two-Photon Laser-Scanning Microscopy.

Methods in molecular biology (Clifton, N.J.)
  • Yuhki Koike
  • ,
  • Bo Li
  • ,
  • Yong Chen
  • ,
  • Hiromu Miyake
  • ,
  • Carol Lee
  • ,
  • Lijun Chi
  • ,
  • Richard Wu
  • ,
  • Mikihiro Inoue
  • ,
  • Keiichi Uchida
  • ,
  • Masato Kusunoki
  • ,
  • Paul Delgado-Olguin
  • ,
  • Agostino Pierro

1752
開始ページ
63
終了ページ
69
記述言語
英語
掲載種別
DOI
10.1007/978-1-4939-7714-7_6

The processes by which the intra-abdominal organ circulatory system develops in the embryo and during organogenesis are unclear. Previous studies have used fixed tissues to study the development of abdominal organ vasculature in the embryo; however, the intravital circulation of intra-abdominal organs in rodent fetal development has not been studied. This protocol describes a system that uses two-photon laser-scanning microscopy (TPLSM) for real-time observation and quantification of normal and pathologic live fetal intra-abdominal dynamics while the fetus is still connected to the mother via the umbilical cord.

リンク情報
DOI
https://doi.org/10.1007/978-1-4939-7714-7_6
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29564762

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