2018年
Live Imaging of Fetal Intra-abdominal Organs Using Two-Photon Laser-Scanning Microscopy.
Methods in molecular biology (Clifton, N.J.)
- 巻
- 1752
- 号
- 開始ページ
- 63
- 終了ページ
- 69
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1007/978-1-4939-7714-7_6
The processes by which the intra-abdominal organ circulatory system develops in the embryo and during organogenesis are unclear. Previous studies have used fixed tissues to study the development of abdominal organ vasculature in the embryo; however, the intravital circulation of intra-abdominal organs in rodent fetal development has not been studied. This protocol describes a system that uses two-photon laser-scanning microscopy (TPLSM) for real-time observation and quantification of normal and pathologic live fetal intra-abdominal dynamics while the fetus is still connected to the mother via the umbilical cord.
- リンク情報
- ID情報
-
- DOI : 10.1007/978-1-4939-7714-7_6
- ISSN : 1064-3745
- PubMed ID : 29564762