2018年8月
Sequence-specific 5mC detection in live cells based on the TALE-split luciferase complementation system
ANALYST
- ,
- ,
- ,
- 巻
- 143
- 号
- 16
- 開始ページ
- 3793
- 終了ページ
- 3797
- 記述言語
- 英語
- 掲載種別
- 研究論文（学術雑誌）
- DOI
- 10.1039/c8an00562a
- 出版者・発行元
- ROYAL SOC CHEMISTRY
We established a method for converting TALE-DNA binding to luminescence, by combining a TALE and a split luciferase system. Furthermore, using a methylation-sensitive TALE, sequence-specific 5mC detection of genomic DNA was achieved in live cells. This study provides a new strategy for exploring the biological functions of 5mC.
- リンク情報
- ID情報
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- DOI : 10.1039/c8an00562a
- ISSN : 0003-2654
- eISSN : 1364-5528
- PubMed ID : 30051128
- Web of Science ID : WOS:000442262000004