2003年6月
DNA cleavage characteristics of non-protein enediyne antibiotic N1999A2
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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- 巻
- 306
- 号
- 1
- 開始ページ
- 87
- 終了ページ
- 92
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/S0006-291X(03)00925-2
- 出版者・発行元
- ACADEMIC PRESS INC ELSEVIER SCIENCE
N1999A2 (NA2) is a new non-protein antitumor antibiotic that contains a stable 9-membered ring enediyne chromophore similar to a neocarzinostatin chromophore (NCS-chr). We have compared DNA cleavage reactions between NA2 and NCS-chr, and also clarified some characteristics of DNA strand scission by NA2. It was found that: (1) NA2 is considerably stable in nature, (2) the compound intercalates into base pairs of a DNA minor groove and decreases its base-attacking frequency in the order of T>A>> C>G, (3) the base-sequence specificity 5'-GGT/3'-CCA presented by NA2 is significantly related to recognition of the base pair with the naphthoate moiety, and (4) the different cleavage property between NCS-chr and NA2 is associated with the presence or absence of an aminoglycoside residue. Based on the results of the site-specific cleavage by NA2 for certain bulged DNAs and a fluorescence study of NA2-DNA oligomer complexes, the DNA interaction mode of NA2 has also been examined. These results provide important information to design a new enediyne molecule for a DNA target. (C) 2003 Elsevier Science (USA). All rights reserved.
- リンク情報
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- DOI
- https://doi.org/10.1016/S0006-291X(03)00925-2
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902225437453342
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/12788070
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000183676900014&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?eid=2-s2.0-0038054267&partnerID=MN8TOARS
- URL
- http://orcid.org/0000-0002-4172-0966
- ID情報
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- DOI : 10.1016/S0006-291X(03)00925-2
- ISSN : 0006-291X
- J-Global ID : 200902225437453342
- ORCIDのPut Code : 44910707
- PubMed ID : 12788070
- SCOPUS ID : 0038054267
- Web of Science ID : WOS:000183676900014