2009年5月
Cytosolic Targeting of Macromolecules Using a pH-Dependent Fusogenic Peptide in Combination with Cationic Liposomes
BIOCONJUGATE CHEMISTRY
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- 巻
- 20
- 号
- 5
- 開始ページ
- 953
- 終了ページ
- 959
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/bc800530v
- 出版者・発行元
- AMER CHEMICAL SOC
pH-Sensitive peptides and polymers have been employed as additives to enhance the cytosolic delivery of drugs and genes by facilitating their endosomal escape. However, little attention has been paid to the intracellular fate of these peptides and polymers. In this study, we explored the possibility of utilizing GALA, a pH-sensitive fusogenic peptide, as a cytosol-targeting vehicle. In combination with cationic liposomes, Lipofectamine 2000 (LF2000), the feasibility of this approach for the cytosolic targeting of proteins and nanoparticles was exemplified through the delivery of avidin (68 kDa) and streptavidin-coated quantum dots (15-20 nm) in serum-containing medium. The use of cationic liposomes is critical to enhance the cell-surface adhesion of the GALA conjugates and eventual endosomal uptake. Circular dichroism studies suggest that the GALA can be liberated from cationic liposomes at a reducing pH to form a helical structure and this may eventually lead to disruption of the endosomal membrane to achieve an efficient leakage of the GALA conjugates into the cytosol.
- リンク情報
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- DOI
- https://doi.org/10.1021/bc800530v
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902259962993864
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/19388672
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000266292500015&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?eid=2-s2.0-66149186868&partnerID=MN8TOARS
- URL
- http://orcid.org/0000-0002-4172-0966
- ID情報
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- DOI : 10.1021/bc800530v
- ISSN : 1043-1802
- J-Global ID : 200902259962993864
- ORCIDのPut Code : 44910705
- PubMed ID : 19388672
- SCOPUS ID : 66149186868
- Web of Science ID : WOS:000266292500015