論文

査読有り 国際誌
2013年

Automated parallel recordings of topologically identified single ion channels.

Scientific reports
  • Ryuji Kawano
  • ,
  • Yutaro Tsuji
  • ,
  • Koji Sato
  • ,
  • Toshihisa Osaki
  • ,
  • Koki Kamiya
  • ,
  • Minako Hirano
  • ,
  • Toru Ide
  • ,
  • Norihisa Miki
  • ,
  • Shoji Takeuchi

3
開始ページ
1995
終了ページ
1995
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/srep01995

Although ion channels are attractive targets for drug discovery, the systematic screening of ion channel-targeted drugs remains challenging. To facilitate automated single ion-channel recordings for the analysis of drug interactions with the intra- and extracellular domain, we have developed a parallel recording methodology using artificial cell membranes. The use of stable lipid bilayer formation in droplet chamber arrays facilitated automated, parallel, single-channel recording from reconstituted native and mutated ion channels. Using this system, several types of ion channels, including mutated forms, were characterised by determining the protein orientation. In addition, we provide evidence that both intra- and extracellular amyloid-beta fragments directly inhibit the channel open probability of the hBK channel. This automated methodology provides a high-throughput drug screening system for the targeting of ion channels and a data-intensive analysis technique for studying ion channel gating mechanisms.

リンク情報
DOI
https://doi.org/10.1038/srep01995
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23771282
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3683667
ID情報
  • DOI : 10.1038/srep01995
  • PubMed ID : 23771282
  • PubMed Central 記事ID : PMC3683667

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