論文

国際誌
2023年8月

Glucose controls manganese homeostasis through transcription factors regulating known and newly identified manganese transporter genes in Bacillus subtilis.

The Journal of biological chemistry
  • Mitsuo Ogura
  • ,
  • Minenosuke Matsutani
  • ,
  • Kei Asai
  • ,
  • Michio Suzuki

299
8
開始ページ
105069
終了ページ
105069
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.jbc.2023.105069

Mn2+ is an essential nutrient whose concentration is tightly controlled in bacteria. In B. subtilis, the Mn2+-activated transcription factor MntR controls Mn2+ transporter genes. However, factors regulating intracellular Mn2+ concentration are incompletely understood. Here, we found that glucose addition induces an increase in intracellular Mn2+ concentration. We determined this upshift was mediated by glucose induction (GI) of the major Mn2+ importer gene mntH by the transcription factor AhrC, which is known to be involved in arginine metabolism and to be indirectly induced by glucose. In addition, we identified novel AhrC-regulated genes encoding the Mn2+ importer YcsG and the ABC-type exporter YknUV. We found the expression of these genes was also regulated by glucose and contributes to the GI of Mn2+ concentrations. ycsG expression is regulated by MntR as well. Furthermore, we analyzed the interaction of AhrC and MntR with the promoter driving ycsG expression and examined the Mn2+-dependent induction of this promoter to identify the transcription factors responsible for the Mn2+ induction. RNA-Seq revealed that disruption of ahrC and mntR affected the expression of 502 and 478 genes, respectively (FDR, <0.001, log2[FC], 2). The AhrC- and/or MntR-dependent expression of twenty promoters was confirmed by LacZ analysis, and AhrC or MntR binding to some of these promoters was observed via electromobility shift assay. The finding that glucose promotes an increase in intracellular Mn2+ levels without changes in extracellular Mn2+ concentrations is reasonable for the bacterium, as intracellular Mn2+ is required for enzymes and pathways mediating glucose metabolism.

リンク情報
DOI
https://doi.org/10.1016/j.jbc.2023.105069
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/37468100
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10448178
ID情報
  • DOI : 10.1016/j.jbc.2023.105069
  • PubMed ID : 37468100
  • PubMed Central 記事ID : PMC10448178

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