論文

2023年6月22日

Stepwise metabolic engineering of Corynebacterium glutamicum for the production of phenylalanine.

The Journal of general and applied microbiology
  • Naoya Kataoka
  • ,
  • Minenosuke Matsutani
  • ,
  • Kazunobu Matsushita
  • ,
  • Toshiharu Yakushi

69
1
開始ページ
11
終了ページ
23
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.2323/jgam.2022.08.002

Corynebacterium glutamicum was metabolically engineered to produce phenylalanine, a valuable aromatic amino acid that can be used as a raw material in the food and pharmaceutical industries. First, a starting phenylalanine-producer was constructed by overexpressing tryptophan-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase and phenylalanine- and tyrosine-insensitive bifunctional enzyme chorismate mutase prephenate dehydratase from Escherichia coli, followed by the inactivation of enzymes responsible for the formation of dihydroxyacetone and the consumption of shikimate pathway-related compounds. Second, redirection of the carbon flow from tyrosine to phenylalanine was attempted by deleting of the tyrA gene encoding prephenate dehydrogenase, which catalyzes the committed step for tyrosine biosynthesis from prephenate. However, suppressor mutants were generated, and two mutants were isolated and examined for phenylalanine production and genome sequencing. The suppressor mutant harboring an amino acid exchange (L180R) on RNase J, which was experimentally proven to lead to a loss of function of the enzyme, showed significantly enhanced production of phenylalanine. Finally, modifications of phosphoenolpyruvate-pyruvate metabolism were investigated, revealing that the inactivation of either phosphoenolpyruvate carboxylase or pyruvate carboxylase, which are enzymes of the anaplerotic pathway, is an effective means for improving phenylalanine production. The resultant strain, harboring a phosphoenolpyruvate carboxylase deficiency, synthesized 50.7 mM phenylalanine from 444 mM glucose. These results not only provided new insights into the practical mutations in constructing a phenylalanine-producing C. glutamicum but also demonstrated the creation of a potential strain for the biosynthesis of phenylalanine-derived compounds represented by plant secondary metabolites.

リンク情報
DOI
https://doi.org/10.2323/jgam.2022.08.002
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/35989300
ID情報
  • DOI : 10.2323/jgam.2022.08.002
  • PubMed ID : 35989300

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