論文

査読有り
2013年6月

Blast resistance of CC-NB-LRR protein Pb1 is mediated by WRKY45 through protein-protein interaction

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
  • Haruhiko Inoue
  • ,
  • Nagao Hayashi
  • ,
  • Akane Matsushita
  • ,
  • Liu Xinqiong
  • ,
  • Akira Nakayama
  • ,
  • Shoji Sugano
  • ,
  • Chang-Jie Jiang
  • ,
  • Hiroshi Takatsuji

110
23
開始ページ
9577
終了ページ
9582
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1073/pnas.1222155110
出版者・発行元
NATL ACAD SCIENCES

Panicle blast 1 (Pb1) is a panicle blast resistance gene derived from the indica rice cultivar "Modan." Pb1 encodes a coiled-coil-nucleotide-binding site-leucine-rich repeat (CC-NB-LRR) protein and confers durable, broad-spectrum resistance to Magnaporthe oryzae races. Here, we investigated the molecular mechanisms underlying Pb1-mediated blast resistance. The Pb1 protein interacted with WRKY45, a transcription factor involved in induced resistance via the salicylic acid signaling pathway that is regulated by the ubiquitin proteasome system. Pb1-mediated panicle blast resistance was largely compromised when WRKY45 was knocked down in a Pb1-containing rice cultivar. Leaf-blast resistance by Pb1 overexpression (Pb1-ox) was also compromised in WRKY45 knock-down/Pb1-ox rice. Blast infection induced higher accumulation of WRKY45 in Pb1-ox than in control Nipponbare rice. Overexpression of Pb1-Quad, a coiled-coil domain mutant that had weak interaction with WRKY45, resulted in significantly weaker blast resistance than that of wild-type Pb1. Overexpression of Pb1 with a nuclear export sequence failed to confer blast resistance to rice. These results suggest that the blast resistance of Pb1 depends on its interaction with WRKY45 in the nucleus. In a transient system using rice protoplasts, coexpression of Pb1 enhanced WRKY45 accumulation and increased WRKY45-dependent transactivation activity, suggesting that protection of WRKY45 from ubiquitin proteasome system degradation is possibly involved in Pb1-dependent blast resistance.

Web of Science ® 被引用回数 : 112

リンク情報
DOI
https://doi.org/10.1073/pnas.1222155110
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000320503000081&DestApp=WOS_CPL
ID情報
  • DOI : 10.1073/pnas.1222155110
  • ISSN : 0027-8424
  • Web of Science ID : WOS:000320503000081

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