論文

査読有り
2018年6月19日

The PP2A-like Protein Phosphatase Ppg1 and the Far Complex Cooperatively Counteract CK2-Mediated Phosphorylation of Atg32 to Inhibit Mitophagy

Cell Reports
  • Kentaro Furukawa
  • ,
  • Tomoyuki Fukuda
  • ,
  • Shun-ichi Yamashita
  • ,
  • Tetsu Saigusa
  • ,
  • Yusuke Kurihara
  • ,
  • Yutaka Yoshida
  • ,
  • Hiromi Kirisako
  • ,
  • Hitoshi Nakatogawa
  • ,
  • Tomotake Kanki

23
12
開始ページ
3579
終了ページ
3590
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.celrep.2018.05.064
出版者・発行元
Elsevier B.V.

Mitophagy plays an important role in mitochondrial quality control. In yeast, phosphorylation of the mitophagy receptor Atg32 by casein kinase 2 (CK2) upon induction of mitophagy is a prerequisite for interaction of Atg32 with Atg11 (an adaptor protein for selective autophagy) and following delivery of mitochondria to the vacuole for degradation. Because CK2 is constitutively active, Atg32 phosphorylation must be precisely regulated to prevent unrequired mitophagy. We found that the PP2A (protein phosphatase 2A)-like protein phosphatase Ppg1 was essential for dephosphorylation of Atg32 and inhibited mitophagy. We identified the Far complex proteins, Far3, Far7, Far8, Far9, Far10, and Far11, as Ppg1-binding proteins. Deletion of Ppg1 or Far proteins accelerated mitophagy. Deletion of a cytoplasmic region (amino acid residues 151–200) of Atg32 caused the same phenotypes as in ppg1Δ cells, which suggested that dephosphorylation of Atg32 by Ppg1 required this region. Therefore, Ppg1 and the Far complex cooperatively dephosphorylate Atg32 to prevent excessive mitophagy. Mitophagy in yeast is initiated by CK2-mediated phosphorylation of the mitophagy receptor Atg32. However, how this phosphorylation is prevented under non-mitophagy-inducing conditions is unclear. Furukawa et al. show that the PP2A-like protein phosphatase Ppg1 and the Far complex negatively regulate mitophagy by counteracting CK2-mediated phosphorylation of Atg32.

リンク情報
DOI
https://doi.org/10.1016/j.celrep.2018.05.064
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29925000
ID情報
  • DOI : 10.1016/j.celrep.2018.05.064
  • ISSN : 2211-1247
  • PubMed ID : 29925000
  • SCOPUS ID : 85048330078

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