論文

査読有り
2017年7月

Versican Promotes Tumor Progression, Metastasis and Predicts Poor Prognosis in Renal Carcinoma

MOLECULAR CANCER RESEARCH
  • Yozo Mitsui
  • ,
  • Hiroaki Shiina
  • ,
  • Taku Kato
  • ,
  • Shigekatsu Maekawa
  • ,
  • Yutaka Hashimoto
  • ,
  • Marisa Shiina
  • ,
  • Mitsuho Imai-Sumida
  • ,
  • Priyanka Kulkarni
  • ,
  • Pritha Dasgupta
  • ,
  • Ryan Kenji Wong
  • ,
  • Miho Hiraki
  • ,
  • Naoko Arichi
  • ,
  • Shinichiro Fukuhara
  • ,
  • Soichiro Yamamura
  • ,
  • Shahana Majid
  • ,
  • Sharanjot Saini
  • ,
  • Guoren Deng
  • ,
  • Rajvir Dahiya
  • ,
  • Koichi Nakajima
  • ,
  • Yuichiro Tanaka

15
7
開始ページ
884
終了ページ
895
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1158/1541-7786.MCR-16-0444
出版者・発行元
AMER ASSOC CANCER RESEARCH

The proteoglycan versican (VCAN) promotes tumor progression and enhances metastasis in several cancers; however, its role in clear cell renal cell carcinoma (ccRCC) remains unknown. Recent evidence suggests that VCAN is an important target of chromosomal 5q gain, one of the most prevalent genetic abnormalities in ccRCC. Thus, we investigated whether VCAN expression is associated with the pathogenesis of ccRCC. VCAN expression was analyzed using three RCC and normal kidney cell lines as well as a clinical cohort of 84 matched ccRCC and normal renal tissues. Functional analyses on growth and progression properties were performed using VCAN-depleted ccRCC cells. Microarray expression profiling was employed to investigate the target genes and biologic pathways involved in VCAN-mediated ccRCC carcinogenesis. ccRCC had elevated VCAN expression in comparison with normal kidney in both cell lines and clinical specimens. The elevated expression of VCAN was significantly correlated with metastasis (P < 0.001) and worse 5year overall survival after radical nephrectomy (P = 0.014). In vitro, VCAN knockdown significantly decreased cell proliferation and increased apoptosis in Caki-2 and 786-O cells, and this was associated with alteration of several TNF signalingrelated genes such as TNF alpha, BID, and BAK. Furthermore, VCAN depletion markedly decreased cell migration and invasion which correlated with reduction of MMP7 and CXCR4. These results demonstrate that VCAN promotes ccRCC tumorigenesis and metastasis and thus is an attractive target for novel diagnostic, prognostic, and therapeutic strategies.
Implications: This study highlights the oncogenic role of VCAN in renal cell carcinogenesis and suggests that this gene has therapeutic and/or biomarker potential for renal cell cancer.

Web of Science ® 被引用回数 : 16

リンク情報
DOI
https://doi.org/10.1158/1541-7786.MCR-16-0444
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000405293700010&DestApp=WOS_CPL