石川充 (Ishikawa Mitsuru) - マイポータル

基本情報

所属: 慶應義塾大学医学部（信濃町）特任助教

学位: 薬学(富山大学)

J-GLOBAL ID: 201301043207447783
researchmap会員ID: B000233575

外部リンク

ヒトの神経細胞を「作る・育てる・見る・操作する」
ことで、さまざまな遺伝性神経難病や神経系common diseaseの
病態解析と創薬を行っています。
特に以下の研究開発、および技術指導・提供を行っています。

1) 疾患特異的iPS細胞技術を用いた神経難病研究
1)-1 精神疾患研究
1)-2 小児神経疾患・てんかん脳症研究
1)-3 認知症研究
1)-4 ALS研究
2) 安定で確実なiPS細胞樹立と品質管理法 / 技術指導
3) スクリーニング向けヒト神経細胞の調製技術
3)-1 超均一「興奮性」神経細胞の迅速作出法 / 技術指導
3)-2 超均一「抑制性」神経細胞の迅速作出法 / 技術指導
3)-3 迅速脳オルガノイド培養法開発
4) iPS細胞を介さない直接的な神経系誘導技術開発
5) ヒト神経細胞を用いた「光操作と光計測」開発
6) 遺伝子エンハンサー同定技術応用（CAGE-SEQ）
7) ヒトiPS細胞へのゲノム編集技術開発と技術指導
8) 霊長類進化とゲノム、脳機能の研究

経歴

2021年4月 - 現在

慶應義塾大学医学部生理学教室特任講師

論文

Generation of a control human induced pluripotent stem cell line using the defective and persistent Sendai virus vector system.

Zhi Zhou, Sho Yoshimatsu, Emi Qian, Mitsuru Ishikawa, Tsukika Sato, Manami Ohtaka, Mahito Nakanishi, Hideyuki Okano

Stem cell research 56 102549-102549 2021年9月23日
Identification of hub molecules of FUS-ALS by Bayesian gene regulatory network analysis of iPSC model: iBRN.

Masahiro Nogami, Mitsuru Ishikawa, Atsushi Doi, Osamu Sano, Takefumi Sone, Tetsuya Akiyama, Masashi Aoki, Atsushi Nakanishi, Kazuhiro Ogi, Masato Yano, Hideyuki Okano

Neurobiology of disease 155 105364-105364 2021年7月
Impaired neuronal activity and differential gene expression in STXBP1 encephalopathy patient iPSC-derived GABAergic neurons.

Eisuke Ichise, Tomohiro Chiyonobu, Mitsuru Ishikawa, Yasuyoshi Tanaka, Mami Shibata, Takenori Tozawa, Yoshihiro Taura, Satoshi Yamashita, Michiko Yoshida, Masafumi Morimoto, Norimichi Higurashi, Toshiyuki Yamamoto, Hideyuki Okano, Shinichi Hirose

Human molecular genetics 30(14) 1337-1348 2021年6月26日
Reduced PHOX2B stability causes axonal growth impairment in motor neurons with TARDBP mutations.

Shio Mitsuzawa, Naoki Suzuki, Tetsuya Akiyama, Mitsuru Ishikawa, Takefumi Sone, Jiro Kawada, Ryo Funayama, Matsuyuki Shirota, Hiroaki Mitsuhashi, Satoru Morimoto, Kensuke Ikeda, Tomomi Shijo, Akiyuki Ohno, Naoko Nakamura, Hiroya Ono, Risako Ono, Shion Osana, Tadashi Nakagawa, Ayumi Nishiyama, Rumiko Izumi, Shohei Kaneda, Yoshiho Ikeuchi, Keiko Nakayama, Teruo Fujii, Hitoshi Warita, Hideyuki Okano, Masashi Aoki

Stem cell reports 16(6) 1527-1541 2021年6月8日
Establishing an induced pluripotent stem cell line from neonatal common marmoset fibroblasts by an all-in-one episomal vector approach.

Sho Yoshimatsu, Emi Qian, Tsukika Sato, Masafumi Yamamoto, Mitsuru Ishikawa, Hideyuki Okano

Stem cell research 53 102380-102380 2021年5月
Non-viral Induction of Transgene-free iPSCs from Somatic Fibroblasts of Multiple Mammalian Species.

Sho Yoshimatsu, Mayutaka Nakajima, Aozora Iguchi, Tsukasa Sanosaka, Tsukika Sato, Mari Nakamura, Ryusuke Nakajima, Eri Arai, Mitsuru Ishikawa, Kent Imaizumi, Hirotaka Watanabe, Junko Okahara, Toshiaki Noce, Yuta Takeda, Erika Sasaki, Rüdiger Behr, Kazuya Edamura, Seiji Shiozawa, Hideyuki Okano

Stem cell reports 16(4) 754-770 2021年4月13日
DCTN1 Binds to TDP-43 and Regulates TDP-43 Aggregation.

Manami Deshimaru, Mariko Kinoshita-Kawada, Kaori Kubota, Takuya Watanabe, Yasuyoshi Tanaka, Saito Hirano, Fumiyoshi Ishidate, Masaki Hiramoto, Mitsuru Ishikawa, Yoshinari Uehara, Hideyuki Okano, Shinichi Hirose, Shinsuke Fujioka, Katsunori Iwasaki, Junichi Yuasa-Kawada, Takayasu Mishima, Yoshio Tsuboi

International journal of molecular sciences 22(8) 2021年4月13日
Generation of region-specific and high-purity neurons from human feeder-free iPSCs.

Tsukika Sato, Kent Imaizumi, Hirotaka Watanabe, Mitsuru Ishikawa, Hideyuki Okano

Neuroscience letters 746 135676-135676 2021年2月16日
Human Astrocytes Model Derived from Induced Pluripotent Stem Cells.

Nicolas Leventoux, Satoru Morimoto, Kent Imaizumi, Yuta Sato, Shinichi Takahashi, Kyoko Mashima, Mitsuru Ishikawa, Iki Sonn, Takahiro Kondo, Hirotaka Watanabe, Hideyuki Okano

Cells 9(12) 2020年12月13日
Generation of gene-corrected iPSCs line (KEIUi001-A) from a PARK8 patient iPSCs with familial Parkinson's disease carrying the I2020T mutation in LRRK2.

Etsuro Ohta, Takefumi Sone, Hideki Ukai, Tomoko Hisamatsu, Tokiko Kitagawa, Mitsuru Ishikawa, Makiko Nagai, Hiroki R Ueda, Fumiya Obata, Hideyuki Okano

Stem cell research 49 102073-102073 2020年12月
Application of induced pluripotent stem cells in epilepsy.

Shinichi Hirose, Yasuyoshi Tanaka, Mami Shibata, Yuichi Kimura, Mitsuru Ishikawa, Norimichi Higurashi, Toshiyuki Yamamoto, Eisuke Ichise, Tomohiro Chiyonobu, Atsushi Ishii

Molecular and cellular neurosciences 108 103535-103535 2020年10月
Differential localization and roles of splice variants of rat suppressor of cancer cell invasion (SCAI) in neuronal cells

Miho Mizukoshi, Ayaka Nozawa, Serina Oomizo, Daisuke Ihara, Jun Shiota, Keietsu Kikuchi, Maki Kaito, Yuta Ishibashi, Mitsuru Ishikawa, Mamoru Fukuchi, Masaaki Tsuda, Ichiro Takasaki, Akiko Tabuchi

Biochemical and Biophysical Research Communications 529(3) 615-621 2020年8月査読有り
Neuron-enriched phosphatase and actin regulator 3 (Phactr3)/ nuclear scaffold-associated PP1-inhibiting protein (Scapinin) regulates dendritic morphology via its protein phosphatase 1-binding domain.

Tomoaki Miyata, Keietsu Kikuchi, Daisuke Ihara, Maki Kaito, Yuta Ishibashi, Tomoyuki Hakamata, Tetsuya Yamada, Mitsuru Ishikawa, Miho Mizukoshi, Shizuku Shoji, Mamoru Fukuchi, Masaaki Tsuda, Yamato Hida, Toshihisa Ohtsuka, Marisa Kaneda, Akiko Tabuchi

Biochemical and biophysical research communications 528(2) 322-329 2020年5月15日査読有り
Brain Transcriptome Analysis Links Deficiencies of Stress-Responsive Proteins to the Pathomechanism of Kii ALS/PDC.

Satoru Morimoto, Mitsuru Ishikawa, Hirotaka Watanabe, Miho Isoda, Masaki Takao, Shiho Nakamura, Fumiko Ozawa, Yoshifumi Hirokawa, Shigeki Kuzuhara, Hideyuki Okano, Yasumasa Kokubo

Antioxidants (Basel, Switzerland) 9(5) 2020年5月14日査読有り
miRNA-Based Rapid Differentiation of Purified Neurons from hPSCs Advancestowards Quick Screening for Neuronal Disease Phenotypes In Vitro.

Mitsuru Ishikawa, Takeshi Aoyama, Shoichiro Shibata, Takefumi Sone, Hiroyuki Miyoshi, Hirotaka Watanabe, Mari Nakamura, Saori Morota, Hiroyuki Uchino, Andrew S Yoo, Hideyuki Okano

Cells 9(3) 532 2020年2月25日査読有り筆頭著者
A combinational treatment of carotenoids decreases Aβ secretion in human neurons via β-secretase inhibition.

Sho M, Ichiyanagi N, Imaizumi K, Ishikawa M, Morimoto S, Watanabe H, Okano H

Neuroscience research 158 47-55 2019年10月10日査読有り
In vitro modeling of the bipolar disorder and schizophrenia using patient-derived induced pluripotent stem cells with copy number variations of PCDH15 and RELN.

Ishii T, Ishikawa M, Fujimori K, Maeda T, Kushima I, Arioka Y, Mori D, Nakatake Y, Yamagata B, Nio S, Kato TA, Yang N, Wernig M, Kanba S, Mimura M, Ozaki N, Okano H

eNeuro 2019年9月査読有り
Aberrant axon branching via Fos-B dysregulation in FUS-ALS motor neurons.

Tetsuya Akiyama, Naoki Suzuki, Mitsuru Ishikawa, Koki Fujimori, Takefumi Sone, Jiro Kawada, Ryo Funayama, Fumiyoshi Fujishima, Shio Mitsuzawa, Kensuke Ikeda, Hiroya Ono, Tomomi Shijo, Shion Osana, Matsuyuki Shirota, Tadashi Nakagawa, Yasuo Kitajima, Ayumi Nishiyama, Rumiko Izumi, Satoru Morimoto, Yohei Okada, Takayuki Kamei, Mayumi Nishida, Masahiro Nogami, Shohei Kaneda, Yoshiho Ikeuchi, Hiroaki Mitsuhashi, Keiko Nakayama, Teruo Fujii, Hitoshi Warita, Hideyuki Okano, Masashi Aoki

EBioMedicine 45 362-378 2019年7月査読有り
Involvement of SRF coactivator MKL2 in BDNF-mediated activation of the synaptic activity-responsive element in the Arc gene.

Keietsu Kikuchi, Daisuke Ihara, Mamoru Fukuchi, Hiroki Tanabe, Yuta Ishibashi, Junya Tsujii, Masaaki Tsuda, Marisa Kaneda, Hiroyuki Sakagami, Hiroyuki Okuno, Haruhiko Bito, Yuya Yamazaki, Mitsuru Ishikawa, Akiko Tabuchi

Journal of neurochemistry 148(2) 204-218 2019年1月査読有り
A versatile toolbox for knock-in gene targeting based on the Multisite Gateway technology.

Sho Yoshimatsu, Takefumi Sone, Mayutaka Nakajima, Tsukika Sato, Ryotaro Okochi, Mitsuru Ishikawa, Mari Nakamura, Erika Sasaki, Seiji Shiozawa, Hideyuki Okano

PloS one 14(8) e0221164 2019年査読有り

もっとみる

MISC

遺伝性パーキンソン病患者iPS細胞由来神経細胞における経時的なmRNA発現解析

太田悦朗, 太田悦朗, 太田悦朗, 太田悦朗, 曽根岳史, 大日方友理, 鵜飼英樹, 久松知子, 北川季子, 石川充, 駒野肇, 上田泰己, 上田泰己, 上田泰己, 小幡文弥, 小幡文弥, 小幡文弥, 川村俊彦, 川村俊彦, 岡野栄之

日本再生医療学会総会(Web) 17th ROMBUNNO.O‐41‐5 (WEB ONLY) 2018年
iPS細胞におけるAngelman症候群原因遺伝子UBE3Aノックアウトの作製と神経細胞の表現型探索

大河内遼太郎, 曽根岳史, 石川充, 奥野博庸, 中武悠樹, 洪実, 小崎健次郎, 岡野栄之

再生医療 16 425 2017年2月1日
遺伝性パーキンソン病患者iPS細胞におけるゲノム編集iPS細胞の樹立

太田悦朗, 太田悦朗, 太田悦朗, 太田悦朗, 曽根岳史, 大日方友理, 鵜飼英樹, 久松知子, 北川季子, 石川充, 石川充, 石川充, 駒野肇, 上田泰己, 上田泰己, 上田泰己, 小幡文弥, 小幡文弥, 小幡文弥, 岡野栄之

再生医療 16 312 2017年2月1日
Studies on the molecular mechanism of clozapine action with iPSC technology

Fujiwara Mikiya, Kikuchi Masataka, Nagayasu Kazuki, Nakazawa Takanobu, Yamamori Hidenaga, Kasai Atsushi, Hayata-Takano Atsuko, Shintani Norihito, Fujimoto Michiko, Yasuda Yuka, Ishikawa Mitsuru, Akamatsu Wado, Okano Hideyuki, Nakaya Akihiro, Hashimoto Hitoshi, Hashimoto Ryota

JOURNAL OF PHARMACOLOGICAL SCIENCES 130(3) S254 2016年3月査読有り
COQ2変異をもつ多系統萎縮症患者由来iPS細胞を用いた病態機序の解明と治療法の検討

中元ふみ子, 中元ふみ子, 岡本理志, 三井純, 駒野肇, 石川充, 辻省次, 岡野栄之

再生医療 15 263 2016年2月1日
iPS細胞を用いた精神疾患の分子病態研究の現状と展望 iPS細胞関連技術を用いた統合失調症研究

中澤敬信, 橋本亮太, 永安一樹, 安田由華, 山森英長, 梅田知美, 藤本美智子, 大井一高, 石川充, 赤松和土, 岡野栄之, 武田雅俊, 橋本均

日本臨床精神神経薬理学会・日本神経精神薬理学会合同年会プログラム・抄録集 24回・44回 121-121 2014年11月
Phosphorylation of the SRF coactivator MKL1 induced by brain-derived neurotrophic factor (BDNF) in rat cortical neurons

Akiko Tabuchi, Yuta Ishibashi, Jyunya Tsujii, Mitsuru Ishikawa, Mamoru Fukuchi, Masaaki Tsuda

NEUROSCIENCE RESEARCH 71 E335-E335 2011年
Developmental expression of the MKL/SRF corepressor SCAI in the brain

Miho Mizukoshi, Mitsuru Ishikawa, Maki Kaito, Mamoru Fukuchi, Masaaki Tsuda, Akiko Tabuchi

NEUROSCIENCE RESEARCH 71 E233-E233 2011年
Actin-binding coactivator MKL is involved in a novel signaling pathway for activin-regulated dendritic complexity of rat cortical neurons

Mitsuru Ishikawa, Naoki Nishijima, Hiroyuki Sakagami, Kunihiro Tsuchida, Miho Mizukoshi, Jun Shiota, Mamoru Fukuchi, Masaaki Tsuda, Akiko Tabuchi

NEUROSCIENCE RESEARCH 71 E333-E333 2011年
Isolation of actin-bound SRF coactivator MKL1 splice variants differentially expressed in the developing rat brain

Akiko Tabuchi, Tomoyuki Hakamata, Mitsuru Ishikawa, Jun Shiota, Shizuku Shoji, Masaaki Tsuda

NEUROSCIENCE RESEARCH 68 E140-E140 2010年
MKL, an actin-binding coactivator for SRF, is involved in activin-induced alteration of dendritic morphology and transcriptional activity in rat cortical neurons

Mitsuru Ishikawa, Naoki Nishijima, Hiroyuki Sakagami, Kunihiro Tsuchida, Masaaki Tsuda, Akiko Tabuchi

NEUROSCIENCE RESEARCH 68 E81-E81 2010年
Functional analysis of MKL2, actin-binding coactivators for SRF, in neuronal morphology and gene expression

Mitsuru Ishikawa, Naoki Nishijima, Hiroyuki Sakagami, Masaaki Tsuda, Akiko Tabuchi

NEUROSCIENCE RESEARCH 61 S82-S82 2008年
Distribution of MKL1/2, actin-binding coactivators for SRF, in developing rodent brain

Mitsuru Ishikawa, Naoki Nishijima, Hiroyuki Sakagami, Masaaki Tsuda, Akiko Tabuchi

NEUROSCIENCE RESEARCH 58 S142-S142 2007年
Functional analysis of MKL1/2, actin-binding coactivators for SRF, in neuronal morphology and gene expression

Naoki Nishijima, Mitsuru Ishikawa, Hiroyuki Tsutsumishita, Jun Shiota, Masaaki Tsuda, Akiko Tabuchi

NEUROSCIENCE RESEARCH 58 S142-S142 2007年
Distribution of the SRF co-activator MAL in developing mouse brain

Mitsuru Ishikawa, Jun Shiota, Hiroyuki Tsutsumishita, Hiroyuki Sakagami, Masaaki Tsuda, Akiko Tabuchi

NEUROSCIENCE RESEARCH 55 S214-S214 2006年