Papers

Peer-reviewed
Jun, 2004

Reversible immobilization of engineered molecules by Ni-NTA chelators

BIOELECTROCHEMISTRY
  • J Maly
    • ,
  • C Di Meo
    • ,
  • M De Francesco
    • ,
  • A Masci
    • ,
  • J Masojidek
    • ,
  • M Sugiura
    • ,
  • A Volpe
    • ,
  • R Pilloton
Volume
63
Number
1-2
First page
271
Last page
275
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1016/j.bioelechem.2003.10.024
Publisher
ELSEVIER SCIENCE SA
Electrochemical synthesis of nickel-nitrilotriacetic acid (Ni-NTA) chelators, for subsequent immobilization of (HiS)(6)-tagged proteins (Photosystem II (PSII) as model molecule), on Au or Au-graphite electrodes is compared to chemical synthesis. Results show: (i) higher Ni-NTA surface density, (ii) shorter treatment time (1-12 min vs. 16 h normally needed for self-assembled monolayer (SAM)), (iii) possibility of addressing the chelator to only one Au electrode, in a sensor mu-array. (C) 2004 Elsevier B.V. All rights reserved.
Link information
DOI
https://doi.org/10.1016/j.bioelechem.2003.10.024
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/15110286
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000221786900049&DestApp=WOS_CPL
ID information
  • DOI : 10.1016/j.bioelechem.2003.10.024
  • ISSN : 1567-5394
  • Pubmed ID : 15110286
  • Web of Science ID : WOS:000221786900049
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