論文

査読有り
2015年11月

EndoQ and EndoV work individually for damaged DNA base repair in Pyrococcus furiosus

BIOCHIMIE
  • Sonoko Ishino
  • ,
  • Naruto Makita
  • ,
  • Miyako Shiraishi
  • ,
  • Takeshi Yamagami
  • ,
  • Yoshizumi Ishino

118
開始ページ
264
終了ページ
269
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.biochi.2015.06.015
出版者・発行元
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER

Base deamination is a typical form of DNA damage, and it must be repaired quickly to maintain the genome integrity of living organisms. Endonuclease Q (EndoQ), recently found in the hyperthermophilic archaea, is an enzyme that cleaves the phosphodiester bond 5' from the damaged nucleotide in the DNA strand, and may primarily function to start the repair process for the damaged bases. Endonuclease V (EndoV) also hydrolyzes the second phosphodiester bond 3' from the damaged nucleotide, although the hyperthermophilic archaeal EndoV is a strictly hypoxanthine-specific endonuclease. To understand the relationships of the EndoQ and EndoV functions in hyperthermophilic archaea, we analyzed their interactions in hypoxanthine repair. EndoQ and EndoV do not directly interact with each other in either the presence or absence of DNA. However, EndoQ and EndoV individually worked on deoxyinosine (dl)-containing DNA at each cleavage site. EndoQ has higher affinity to dl-containing DNA than EndoV, and cells produce higher amounts of EndoQ as compared to EndoV. These data support the proposal that EndoQ primarily functions for, at least, dl-containing DNA. (C) 2015 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.biochi.2015.06.015
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26116888
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000365062200030&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.biochi.2015.06.015
  • ISSN : 0300-9084
  • eISSN : 1638-6183
  • PubMed ID : 26116888
  • Web of Science ID : WOS:000365062200030

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