Ryoji Miyazaki

Profile Information

Affiliation: Institute for Frontier Medical Sciences, Nara Institute of Science and Technology

Degree: 博士（理学）(京都大学)

Researcher number: 30827564
ORCID ID: https://orcid.org/0000-0003-1626-2569
J-GLOBAL ID: 202101013090589731
researchmap Member ID: R000016850

Research Areas

Research History

Mar, 2021 - Present

Nara Institute of Science and Technology
Apr, 2018 - Feb, 2021

Kyoto University

Papers

YeeD is an essential partner for YeeE-mediated thiosulfate uptake in bacteria and regulates thiosulfate ion decomposition

Mai Ikei, Ryoji Miyazaki, Keigo Monden, Yusuke Naito, Azusa Takeuchi, Yutaro S. Takahashi, Yoshiki Tanaka, Keina Murata, Takaharu Mori, Muneyoshi Ichikawa …

PLOS Biology, 22(40) e3002601, Apr 24, 2024 Peer-reviewed
Structure and function of a YeeE-YeeD complex for sophisticated thiosulfate uptake

Mai Ikei, Ryoji Miyazaki, Keigo Monden, Yusuke Naito, Azusa Takeuchi, Yutaro S. Takahashi, Yoshiki Tanaka, Takaharu Mori, Muneyoshi Ichikawa, Tomoya Tsukazaki

Jul 29, 2023
Analyzing protein intermediate interactions in living E. coli cells using site-specific photo-crosslinking combined with chemical crosslinking

Ryoji Miyazaki, Yoshinori Akiyama

STAR Protocols, 4(2) 102178-102178, Jun, 2023 Peer-reviewedLead authorCorresponding author
Critical residues of the antibiotic peptide Lys^Mthat inhibits lipid II flipping

Hidetaka Kohga, Napathip Lertpreedakorn, Ryoji Miyazaki, Tomoya Tsukazaki

May 22, 2023
Inner membrane YfgM-PpiD heterodimer acts as a functional unit that associates with the SecY/E/G translocon and promotes protein translocation.

Ryoji Miyazaki, Mengting Ai, Natsuko Tanaka, Takehiro Suzuki, Naoshi Dhomae, Tomoya Tsukazaki, Yoshinori Akiyama, Hiroyuki Mori

The Journal of biological chemistry, 298(11) 102572-102572, Oct 7, 2022 Peer-reviewedLead author
A Photo-Crosslinking Approach to Monitoring the Assembly of an LptD Intermediate with LptE in a Living Cell.

Ryoji Miyazaki, Hiroyuki Mori, Yoshinori Akiyama

Methods in molecular biology (Clifton, N.J.), 2548 97-107, 2022 Lead author
Edge-strand of BepA interacts with immature LptD on the β-barrel assembly machine to direct it to on- and off-pathways

Ryoji Miyazaki, Tetsuro Watanabe, Kohei Yoshitani, Yoshinori Akiyama

eLife, 10 e70541, Aug, 2021 Peer-reviewedLead author
PiXie Analysis for Monitoring Dynamic Protein Interaction and Folding in a Living Cell

MIYAZAKI Ryoji, MORI Hiroyuki, AKIYAMA Yoshinori

Seibutsu Butsuri, 61(1) 36-39, 2021 Lead author
Fine interaction profiling of VemP and mechanisms responsible for its translocation-coupled arrest-cancelation.

Ryoji Miyazaki, Yoshinori Akiyama, Hiroyuki Mori

eLife, 9 e62623, Dec 15, 2020 Peer-reviewedLead author
Reversible autoinhibitory regulation of Escherichia coli metallopeptidase BepA for selective β-barrel protein degradation.

Yasushi Daimon, Shin-Ichiro Narita, Ryoji Miyazaki, Yohei Hizukuri, Hiroyuki Mori, Yoshiki Tanaka, Tomoya Tsukazaki, Yoshinori Akiyama

Proceedings of the National Academy of Sciences of the United States of America, 117(45) 27989-27996, Nov 10, 2020 Peer-reviewed
A photo-cross-linking approach to monitor protein dynamics in living cells.

Ryoji Miyazaki, Yoshinori Akiyama, Hiroyuki Mori

Biochimica et biophysica acta. General subjects, 1864(2) 129317-129317, Feb, 2020 Peer-reviewedLead author
Heat shock transcription factor σ32 defective in membrane transport can be suppressed by transposon insertion into genes encoding a restriction enzyme subunit or a putative autotransporter in Escherichia coli.

Takashi Yura, Ryoji Miyazaki, Keigo Fujiwara, Koreaki Ito, Shinobu Chiba, Hiroyuki Mori, Yoshinori Akiyama

Genes & genetic systems, 93(6) 229-235, Jan 19, 2019 Peer-reviewed
A photo-cross-linking approach to monitor folding and assembly of newly synthesized proteins in a living cell.

Ryoji Miyazaki, Naomi Myougo, Hiroyuki Mori, Yoshinori Akiyama

The Journal of biological chemistry, 293(2) 677-686, Jan 12, 2018 Peer-reviewedLead author
The TPR domain of BepA is required for productive interaction with substrate proteins and the β-barrel assembly machinery complex.

Yasushi Daimon, Chigusa Iwama-Masui, Yoshiki Tanaka, Takuya Shiota, Takehiro Suzuki, Ryoji Miyazaki, Hiroto Sakurada, Trevor Lithgow, Naoshi Dohmae, Hiroyuki Mori …

Molecular microbiology, 106(5) 760-776, Dec, 2017 Peer-reviewed
A Novel SRP Recognition Sequence in the Homeostatic Control Region of Heat Shock Transcription Factor σ32.

Ryoji Miyazaki, Takashi Yura, Takehiro Suzuki, Naoshi Dohmae, Hiroyuki Mori, Yoshinori Akiyama

Scientific reports, 6 24147-24147, Apr 7, 2016 Peer-reviewedLead author
Heat shock transcription factor σ32 co-opts the signal recognition particle to regulate protein homeostasis in E. coli.

Bentley Lim, Ryoji Miyazaki, Saskia Neher, Deborah A Siegele, Koreaki Ito, Peter Walter, Yoshinori Akiyama, Takashi Yura, Carol A Gross

PLoS biology, 11(12) e1001735, Dec, 2013 Peer-reviewed
Transcription regulation system mediated by mechanical operation of a DNA nanostructure.

Masayuki Endo, Ryoji Miyazaki, Tomoko Emura, Kumi Hidaka, Hiroshi Sugiyama

Journal of the American Chemical Society, 134(6) 2852-5, Feb 15, 2012 Peer-reviewed

Misc.

改良型部位特異的in vivo光架橋法による生細胞内での翻訳途上ポリペプチド鎖の相互作用動態解析

宮崎亮次, 秋山芳展, 森博幸

日本生化学会大会プログラム・講演要旨集, 92回 [2P-076], Sep, 2019
大腸菌表層ストレス応答:インプットとアウトプットに働くプロテアーゼの機能

秋山芳展, 秋山光市郎, 岩間(舛井)千草, 大門康志, 檜作洋平, 水野慎也, 宮崎亮次, 森博幸, 禾晃和, 塩田拓也 …

Institute for Fermentation, Osaka. Research Communications, (33), 2019
大腸菌のペリプラズムプロテアーゼBepAは,TPR domainを介したタンパク質間相互作用により外膜機能を維持する

大門康志, 舛井千草, 宮崎亮次, 櫻田洋人, 田中良樹, 鈴木健裕, 堂前直, 森博幸, 塚崎智也, 成田新一郎 …

日本農芸化学会大会講演要旨集(Web), 2017, 2017
部位特異的in vivo光架橋法を用いた生細胞内の動的タンパク質間相互作用解析

森博幸, 宮崎亮次, 町田裕紀子, 三登一八, 何あゆみ, 秋山光市郎, 大門康志, 舛井千草, 塚崎智也, 堂前直 …

日本蛋白質科学会年会プログラム・要旨集, 16th, 2016

Research Projects

特殊な硫黄間相互作用が要となる新奇チオ硫酸トランスポーターの解析

科学研究費助成事業学術変革領域研究(A), 学術変革領域研究(A), 日本学術振興会, Jun, 2022 - Mar, 2024

宮崎亮次
生細胞内での膜透過途上の新生ポリペプチド鎖の相互作用動態解析

科学研究費助成事業若手研究, 若手研究, 日本学術振興会, Apr, 2022 - Mar, 2024

宮崎亮次
Optimization of multiple method baced on neutron reflectometry for analysis of biogenesis of Gram-negative bacterial membrane.

Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)), Fund for the Promotion of Joint International Research (Fostering Joint International Research (B)), Japan Society for the Promotion of Science, Oct, 2021 - Mar, 2024
in vivo光架橋法による生細胞内での新生ポリペプチド鎖の迅速な動態変化の解析

科学研究費助成事業若手研究, 若手研究, 日本学術振興会, Apr, 2020 - Mar, 2022

宮崎亮次
Analysis of dynamic interaction and folding process of a nascent polypeptide in living cells

Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up, Grant-in-Aid for Research Activity Start-up, Japan Society for the Promotion of Science, Apr, 2019 - Mar, 2020

Miyazaki Ryoji
熱ショック転写因子σ３２の膜への輸送を介した新奇な機能制御機構

科学研究費助成事業特別研究員奨励費, 特別研究員奨励費, 日本学術振興会, Apr, 2015 - Mar, 2017

宮﨑亮次

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