論文

査読有り 国際誌
2015年11月16日

Triggering of high-speed neurite outgrowth using an optical microheater.

Scientific reports
  • Kotaro Oyama
  • ,
  • Vadim Zeeb
  • ,
  • Yuki Kawamura
  • ,
  • Tomomi Arai
  • ,
  • Mizuho Gotoh
  • ,
  • Hideki Itoh
  • ,
  • Takeshi Itabashi
  • ,
  • Madoka Suzuki
  • ,
  • Shin'ichi Ishiwata

5
開始ページ
16611
終了ページ
16611
記述言語
英語
掲載種別
DOI
10.1038/srep16611

Optical microheating is a powerful non-invasive method for manipulating biological functions such as gene expression, muscle contraction, and cell excitation. Here, we demonstrate its potential usage for regulating neurite outgrowth. We found that optical microheating with a water-absorbable 1,455-nm laser beam triggers directional and explosive neurite outgrowth and branching in rat hippocampal neurons. The focused laser beam under a microscope rapidly increases the local temperature from 36 °C to 41 °C (stabilized within 2 s), resulting in the elongation of neurites by more than 10 μm within 1 min. This high-speed, persistent elongation of neurites was suppressed by inhibitors of both microtubule and actin polymerization, indicating that the thermosensitive dynamics of these cytoskeletons play crucial roles in this heat-induced neurite outgrowth. Furthermore, we showed that microheating induced the regrowth of injured neurites and the interconnection of neurites. These results demonstrate the efficacy of optical microheating methods for the construction of arbitrary neural networks.

リンク情報
DOI
https://doi.org/10.1038/srep16611
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26568288
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4645119