論文

査読有り
2018年7月

Bone marrow mesenchymal stromal cells protect allograft lung transplants from acute rejection via the PD-L1/IL-17A axis.

Surgery today
  • Naoya Ishibashi
  • ,
  • Tatsuaki Watanabe
  • ,
  • Masahiko Kanehira
  • ,
  • Yui Watanabe
  • ,
  • Yasushi Hoshikawa
  • ,
  • Hirotsugu Notsuda
  • ,
  • Masafumi Noda
  • ,
  • Akira Sakurada
  • ,
  • Shinya Ohkouchi
  • ,
  • Takashi Kondo
  • ,
  • Yoshinori Okada

48
7
開始ページ
726
終了ページ
734
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00595-018-1643-x

PURPOSE: Using a rat model of allograft lung transplantation, we investigated the effectiveness of mesenchymal stromal cells (MSCs) as prophylactic and therapeutic agents against the acute rejection of lung grafts. METHODS: Lung grafts were harvested from donor rats and transplanted orthotopically into major histocompatibility complex-mismatched rats. MSCs were administered to the recipients once (on day 0) or twice (on days 0 and 3) after transplantation. The grade of acute rejection was evaluated both macroscopically and microscopically 6 days after transplantation. To elucidate the related mechanism, mRNA levels of inflammatory cytokines and immunomodulatory receptors in the transplanted grafts were measured using quantitative RT-PCR. RESULTS: The lung graft tissue from the rats that received MSCs post-surgically was protected from acute rejection significantly better than that from the untreated controls. Notably, the rats administered MSCs twice after surgery exhibited the least signs of rejection, with a markedly upregulated mRNA level of PD-L1 and a downregulated mRNA level of IL-17A. CONCLUSION: This study assessed MSC protection of lung allografts from acute rejection by modulating T cell activity via enforced expression of PD-L1 in transplants and downregulation of IL-17A.

リンク情報
DOI
https://doi.org/10.1007/s00595-018-1643-x
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29546496

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